Molecular Analyses
The representative sequences used in this study for phylogenetic
comparisons and molecular analyses were obtained from the Global
Initiative on Sharing Avian Influenza Data (www.gisaid.org) and the
GenBank database (www.ncbi.nlm.nih.gov). The Influenza Virus Sequence
Annotation Tool (https://www.ncbi.nlm.nih.gov/genomes/FLU/annotation/)
was used to predict protein sequences encoded by the input sequences.
The potential N-glycosylation sites were predicted using
the
NetNGlyc 1.0 web server (http://www.cbs.dtu.dk/services/NetNGlyc/). By
default, predictions were carried out only on the Asn-Xaa-Ser/Thr
sequons.