Genome Sequencing and Phylogenetic Analysis
The PCR products were purified with a Wizard DNA Clean-Up System
(Promega), then sequenced using the BigDye Terminator v3.1 Cycle
Sequencing Kit (AB) and analyzed with an ABI 3730XL DNA Analyzer. The
BioEdit program (version 7.1.3.0) and ClustalW alignment algorithm
(version 1.83) were initially used to compare and align nucleotide
sequences. Phylogenetic trees were inferred using the neighbor-joining
method with MEGA software (version 7.0.26), and bootstrap values were
tested with 1000 replications[16]. The
evolutionary distances were computed using the Maximum Composite
Likelihood method and the units represent the number of base
substitutions per site.