3.4.1 Intra-assay variability
To assess the intra-assay variability of the results of the MSD multiplex immunoassay, each sample was added to the plate in duplicate, for both measurements of individual allergens and mixes of allergens. The correlation between the duplicate measures was calculated for all subjects (Table 4). The results show a very stable reproducibility for all samples (r =0.95-1; P<0.001).