3.4.4 Comparison incubation overnight or 2 hours
The MSD multiplex immunoassay protocol states that after adding the standard series and samples, either an incubation overnight at 4°C or for 2 hours at room temperature while shaking the plate, can be applied. To compare the two different incubation periods, the assay was performed in both ways for the individual allergens (Table 4). Correlations were very high (r =0.91-1; P<0.001), meaning that both an incubation overnight as well as for 2 hours can be applied.