2.2.2 Chemical compositions
Fatty acid profiles and minor components (tocopherols, total sterols and
squalene) were performed as described by our previous reports (Yu et
al., 2019). Detailed information associated with the methods used for
fatty acid, tocopherols, total sterols and squalene were all presented
in the supplementary material.
Phenolic compounds were extracted as described by (Mateos et al., 2001).
A 500 mg Sepax Generik Diol tube (Sepax Technologies, Inc., Newark, DE,
USA) was used to extract phenolic compounds. The analysis of phenolic
compounds was performed by a Waters 1525 HPLC system (Milford, MA, USA)
equipped with a Waters 2996 photodiode array detector (Milford, MA, USA)
was used. The stationary phase was a Sepax Amethyst C18-H column (250 ×
4.6 i.d. mm, 5μm particle size) (Sepax Technologies, Inc., Newark, DE,
USA). The mobile phase and solvent gradient conditions used for analysis
were according to (IOC, 2009) with some minor modifications. The flow
rate was 0.8 mL/min. The quantification of phenolic compounds was
carried out at 280 nm.