Conclusion
The identification of potential allele frequency differences among familial crosses can link specific genotypes to important fitness traits. Although only a small number of crosses are presented in this pilot study produced from the mixing of five adult colonies, our findings suggest that selective breeding has measurable effects on the genomic diversity of the resulting offspring. This was apparent in the significant differences in genetic variants and phenotypes associated with stress tolerance when exposed to varying temperatures. These results are in line with studies demonstrating the influence of recent immigrants having significant influence on allele frequency shifts.