Single Nucleotide Polymorphism annotation
SNPs significantly contributing to the PCoA separation between each
family cross were identified, translated to nucleotide sequences by
searching and aligning sequences to the Acropora digitiferagenome (v. OIST_v1.1) using custom scripts. All nucleotide matches were
downloaded and re-formatted into a searchable BLAST database using the
“blastdbcmd” command. Nucleotide matches were blasted to annotated
proteins (OIST v.0.9, see reference for full instructions on
annotations). Predicted proteins were filtered for only protein matches
with E-values <1e-4 were retained
(https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=
blastx&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome).