2.2. Drugs and materials
α-Aminovalerophenone derivatives were synthetized in racemic form as
hydrochloride salts as described below in section 2.2.1. Synthesis
of α-aminovalerophenone derivatives . Solutions for injection were
freshly prepared daily in isotonic saline solution (0.9% NaCl, pH 7.4).
[3H]DA, [3H]5-HT,
[3H]imipramine and
[3H]WIN35,428 were purchased from Perkin Elmer
Inc. (Boston, MA, USA).
[3H]1-Methyl-4-phenylpyridinium
([3H]MPP+), was supplied by
American Radiolabeled Chemicals (St. Louis, USA). Cocaine was generously
provided by the Spanish National Institute of Toxicology. Pargyline,
HEPES sodium and ascorbic acid as well as cell culture media (Dulbecco’s
Modified Eagle’s medium (DMEM) high-glucose) were purchased from
Sigma-Aldrich (St. Louis, MO, USA). Cell culture dishes and 96-well
plates were obtained from Sarstedt (Nuembrecht, Germany). All other
reagents were of analytical grade and purchased from several commercial
sources.