2.2. Drugs and materials
α-Aminovalerophenone derivatives were synthetized in racemic form as hydrochloride salts as described below in section 2.2.1. Synthesis of α-aminovalerophenone derivatives . Solutions for injection were freshly prepared daily in isotonic saline solution (0.9% NaCl, pH 7.4). [3H]DA, [3H]5-HT, [3H]imipramine and [3H]WIN35,428 were purchased from Perkin Elmer Inc. (Boston, MA, USA). [3H]1-Methyl-4-phenylpyridinium ([3H]MPP+), was supplied by American Radiolabeled Chemicals (St. Louis, USA). Cocaine was generously provided by the Spanish National Institute of Toxicology. Pargyline, HEPES sodium and ascorbic acid as well as cell culture media (Dulbecco’s Modified Eagle’s medium (DMEM) high-glucose) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture dishes and 96-well plates were obtained from Sarstedt (Nuembrecht, Germany). All other reagents were of analytical grade and purchased from several commercial sources.