2.4 The role of OPTN in lysosome quality control
Lysosomes are organelles central to degradation and recycling processes
in animal cells. In autophagy, autophagosome fuses with
a lysosome containing degrading enzymes and leads to the digestion of
the autophagosome content. Therefore, although lysosomes often appear as
autolysosome in autophagy, the normal function of lysosomes is very
important to ensure that autophagy proceeds without a hitch. Claudioet al . using high-resolution and live-cell CLEM in AS-stimulated
microglial cells demonstrated that both TBK1 and OPTN are recruitment to
ubiquitylated lysosomes to handle with lysosomal quality impairment and
restore lysosomal quality control by autophagy, which also called
lysophagy named by autophagy substrate(Bussi et al. , 2018). In
general, autophagy induction is frequently associated by up-regulated
hydrolase synthesis and increased lysosome biogenesis(Ghavami et
al. , 2014). When lysosomes are out of stabilizing or their function is
impaired, the autophagy inhibitors tend to lessen the stress of
autophagic on dysfunctional lysosomes by reducing the delivery of
autophagic cargo rather than through an excessively aggressive autophagy
process (Ghavami et al. , 2014). Therefore, the normal function of
lysosomes is important for autophagy.