CD183+CCR7+CD45RA- CD8+Treg inhibit Immunoglobulin production
Purified B cells were cultured alone or with CD8 Treg at and activated with 2ug/ml of anti-human CD40 antibody and 2.5ug/ml of CpG and incubated at 37°C for 14 days. Culture supernatants were collected and IgM, IgG, and IgA measured by ELISA kit against known standards and according manufacturer’s protocol. Figure 2 show data from 6 normal controls at B:CD8 Treg ratio od 1:1. CD8 Treg significantly inhibited IgM (<0.005) and IgG (P<0.039) production; however, inhibition of IgA production was not significant (P>0.078). No significant effect was observed on 1:1/2 and 1:1/4 ratios (data not shown).