2.6 Vaccine PCR and sequencing
The non-structural (NS) and VP2 genes from the FPV Feligen RCP modified
live vaccine (Virbac, France) were amplified and sequenced as previously
described (Pérez et al., 2014; Van Brussel et al., 2019). Amplicons were
sequenced at the AGRF (Melbourne, Australia) and Geneious version
2020.2.5 was used to determine the consensus contig of the NS and VP2
based on the forward and reverse sequence.