Genomic DNA extraction from blood using a bead-based
automated kit
Extraction of genomic DNA from all blood pellet samples with the
bead-based automated kit MagMAX™CORE Nucleic Acid Purification Kit
(ThermoFisher Scientific, Applied Biosystems, USA) was performed
according to the manufacturer’s recommendations. Briefly, 200 µL of
sample was mixed with a bead/Proteinase K mix and a lysis/binding
solution. The mixture was processed using the KingFisher™ Duo Prime
Purification System (ThermoFisher Scientific, USA). Genomic material was
eluted in 90 µl of Elution buffer and stored at -20°C until required.
The concentration and purity of samples were measured with an IMPLEN
Nanophotometer (IMPLEN, Germany).