2.2.2. Pesticide exposure experiments- oxidative stress in
cucumber plants
Single root exposure of CLO, DFN and DFZ:Hydroponic
experiment conditions and plant growth conditions were the same as the“uptake and translocation.” Given the tendency for repeated
application of pesticides, a series of preliminary experiments using
various concentrations of pesticides were carried to evaluated the
cucumber growth parameters. When the root application of CLO at 10mg
L-1, DFN at 20mg L-1 and DFZ at 10mg
L-1, the fresh biomass of cucumber plants
significantly decreased, respectively. We estimated that at these
concentrations, the three pesticides caused significant stress
responses, respectively (Fig S1 ). Therefore, we set the
concentration of CLO at 10 mg L-1, DFN at 20 mg
L-1 and DFZ at 10 mg L-1, and with
SA supplementation at 1mg L-1, 10mg
L-1 and 50mg L-1, the root, stem and
leaf samples were collected after 10 days of growth, respectively. A
total of 12 treatments were set in this part of experiment. The plants
were grown in blank nutrient solution as a control group. After the
root, stem and leaf samples were weighed, the root samples were stored
at -80℃ until measured.