Figure 2. Expression and purification of recombinant TeNT and inactivated decoy molecules . A) SDS-PAGE and Western Blot of purified recombinant TeNT (lanes 1 and 2) and activated di-chain TeNT when non-reduced (lane 3) compared to reduced (lane 4). TeNT was detected at 150 kDa, and activated, reduced TeNT demonstrated the expected 100 kDa heavy-chain and 50 kDa light-chain domains. B)SDS-PAGE and Western blot of TTc R1225E, W1288A (lanes 1 and 2). TTc was detected at 50 kDa as expected. C) SDS-PAGE and Western blot of LCHn (lanes 1 and 2). LCHn was detected at 100 kDa as expected, comprising of the 50 kDa light-chain and Hn regions.