Figure 5. Comparison of tetany development in vaccinated mice when TeNT is administered with different concentrations of decoy solution. A) Comparison of maximum tetany stage reached in 48 hours at different toxin doses with 100 µg of equimolar decoy solution (red triangle), 20 µg of equimolar decoy solution (blue square) and 5 µg of equimolar decoy solution (green circle). B) Comparison of time course of tetany symptoms for mice administered 5 ng of TeNT with 100 µg of equimolar decoy solution (red triangle), 20 µg of equimolar decoy solution (blue square) and 5 µg of equimolar decoy solution (green circle). C) Comparison of time course of tetany symptoms for mice administered 25 ng of TeNT with 100 µg of equimolar decoy solution (red triangle), 20 µg of equimolar decoy solution (blue square) and 5 µg of equimolar decoy solution (green circle). D) Comparison of time course of tetany symptoms for mice administered 500 ng of TeNT with 100 µg of equimolar decoy solution (red triangle), 20 µg of equimolar decoy solution (blue square) and 5 µg of equimolar decoy solution (green circle). Multiple t-test analyses were performed and statistical significance determined using the Holm-Sidak method, with alpha = 0.05. Each dose (A ) or time-point (B-D ) was analysed individually, without assuming a consistent SD. For A , an asterisk denotes a dose point with statistical significance (p < 0.05) between 100 µg and 5 µg of equimolar decoy solution. For B-D , a single asterisk denotes p <0.05 between 100 µg and 5 µg of decoy, a double asterisk denotes p <0.05 between 20 µg and 5µg of decoy and a triple asterisk denotes p <0.05 between 100 µg and 20 µg of decoy. The error bars show the range of tetany stages observed at each time point and where no error bar is displayed, all animals displayed the same stage of tetany.