Purification of decoy proteins
The recombinant decoy proteins (TTc R1225E W1288A and LCHn) were purified individually by NiSO4 IMAC as outlined above, with the exception that 1 mL, gravity fed NTI-sepharose columns (Thermofisher) were used. Additionally, 0.5 mM CHAPS was incorporated into the sample buffer and an additional column wash incorporating 0.5 mM CHAPS was added. Wash and elution buffers included 20 mM and 300 mM imidazole, respectively. Post-IMAC purification the samples were concentrated, and buffer exchanged into PBS using Vivaspin20 columns (30 kDa or 50 kDa MWCO; Sartorious).