Determining protein concentration
The extinction coefficient of the proteins was determined from the
protein sequence using the Protparam tool from Expasy
(https://web.expasy.org/protparam/). The concentration was determined by
absorbance at 280 nm using a Nanodrop One (Thermo Scientific). The final
concentration was determined by the value obtained from the absorbance
divided by the value predicted by the extinction coefficient of a 0.1%
solution.