Figure 4. Comparison of tetany development in vaccinated mice
when TeNT is administered with or without 100 µg of equimolar decoy
solution. A) Maximum stage tetany reached in 48 hours by TeNT dose.
Blue circle is dosage with TeNT and red triangle is dosage with TeNT and
100 µg decoy solution. B) Time course of tetany development for
all doses of TeNT alone. C) Time course of tetany development
for all doses of TeNT with 100 µg decoy solution. D) Comparison
of time course of tetany development of TeNT 500 ng (blue) and TeNT 500
ng decoy 100 µg (red). E) Comparison of time course of tetany
development of TeNT 250 ng (blue) and TeNT 250 ng decoy 100 µg (red).
Multiple t-test analyses were performed and statistical significance
determined using the Holm-Sidak method, with alpha = 0.05. Each time
point was analysed individually, without assuming a consistent SD. An
asterisk denotes a time point with statistical significance (p
< 0.05). The error bars show the range of tetany stages
observed at each time point and where no error bar is displayed, all
animals displayed the same stage of tetany.