Figure 5. Comparison of tetany development in vaccinated mice
when TeNT is administered with different concentrations of decoy
solution. A) Comparison of maximum tetany stage reached in 48 hours at
different toxin doses with 100 µg of equimolar decoy solution (red
triangle), 20 µg of equimolar decoy solution (blue square) and 5 µg of
equimolar decoy solution (green circle). B) Comparison of time
course of tetany symptoms for mice administered 5 ng of TeNT with 100 µg
of equimolar decoy solution (red triangle), 20 µg of equimolar decoy
solution (blue square) and 5 µg of equimolar decoy solution (green
circle). C) Comparison of time course of tetany symptoms for
mice administered 25 ng of TeNT with 100 µg of equimolar decoy solution
(red triangle), 20 µg of equimolar decoy solution (blue square) and 5 µg
of equimolar decoy solution (green circle). D) Comparison of
time course of tetany symptoms for mice administered 500 ng of TeNT with
100 µg of equimolar decoy solution (red triangle), 20 µg of equimolar
decoy solution (blue square) and 5 µg of equimolar decoy solution (green
circle). Multiple t-test analyses were performed and statistical
significance determined using the Holm-Sidak method, with alpha = 0.05.
Each dose (A ) or time-point (B-D ) was analysed
individually, without assuming a consistent SD. For A , an
asterisk denotes a dose point with statistical significance (p
< 0.05) between 100 µg and 5 µg of equimolar decoy solution.
For B-D , a single asterisk denotes p <0.05 between
100 µg and 5 µg of decoy, a double asterisk denotes p <0.05
between 20 µg and 5µg of decoy and a triple asterisk denotes p
<0.05 between 100 µg and 20 µg of decoy. The error bars show
the range of tetany stages observed at each time point and where no
error bar is displayed, all animals displayed the same stage of tetany.