Figure 4. Comparison of tetany development in vaccinated mice when TeNT is administered with or without 100 µg of equimolar decoy solution. A) Maximum stage tetany reached in 48 hours by TeNT dose. Blue circle is dosage with TeNT and red triangle is dosage with TeNT and 100 µg decoy solution. B) Time course of tetany development for all doses of TeNT alone. C) Time course of tetany development for all doses of TeNT with 100 µg decoy solution. D) Comparison of time course of tetany development of TeNT 500 ng (blue) and TeNT 500 ng decoy 100 µg (red). E) Comparison of time course of tetany development of TeNT 250 ng (blue) and TeNT 250 ng decoy 100 µg (red). Multiple t-test analyses were performed and statistical significance determined using the Holm-Sidak method, with alpha = 0.05. Each time point was analysed individually, without assuming a consistent SD. An asterisk denotes a time point with statistical significance (p < 0.05). The error bars show the range of tetany stages observed at each time point and where no error bar is displayed, all animals displayed the same stage of tetany.