Coomassie staining
After electrophoresis, gels were submerged in Coomassie blue stain for
1–3 h. Gels were destained for 1 h in a solution of 40% methanol and
10% acetic acid then destained for a further 1–3 h in a solution of
20% methanol and 10% acetic acid. Following destaining, the gels were
stored in 10% acetic acid.