Determining protein concentration
The extinction coefficient of the proteins was determined from the protein sequence using the Protparam tool from Expasy (https://web.expasy.org/protparam/). The concentration was determined by absorbance at 280 nm using a Nanodrop One (Thermo Scientific). The final concentration was determined by the value obtained from the absorbance divided by the value predicted by the extinction coefficient of a 0.1% solution.