Figure 1. TeNT and decoy molecules and study outline.
A) Representation of the structure of TeNT bound to a synthetic
ganglioside GD1a analogue, derived from x-ray crystallography data (PDB
5N0B, Masuyer et. al. 2017 (Masuyer et al. , 2017)). TeNT
is displayed as a ribbon model, with the TTc domain displayed in red and
orange with the ganglioside GD1a receptor as a grey ball-and-stick
model. The TeNT Hn domain is displayed in yellow and the LC domain is
displayed in blue. B) Diagrammatic representation of the
activation of TeNT from expressed form to di-chain form. C) The
two inactive recombinant protein components of the decoy solution, TTc
fragment with mutated ganglioside binding regions and LCHn. D)Schematic representations of the trial procedure. The vaccinated mouse
procedure is on top and the naïve mouse procedure beneath. E)Example observations of tetany in trial animals demonstrated stage 0–4
tetany from left to right. Stage 0–2 is difficult to differentiate from
still images and are generally observed during animal movement.