Yeast-One Hybrid
Fragments of 60 bp regions of promotors of MtNCED genesMedtr5g025230, Medtr5g025250, Medtr5g02527, predicted to contain a putative binding site for MtHSFA9(http://plantpan2.itps.ncku.edu.tw) (Table S1) were cloned into pBait-AbAi vector as bait constructs. The coding sequence of MtHSFA9 was cloned into the pGADT7-Rec AD cloning vector as prey construct. The empty pGADT7-Rec plasmid was used as negative control. Transformation and screening process were carried out on the media containing SD-Leu and SD-Leu + AbA antibiotic, respectively, according to the manufacturers’ instructions (www.clontech.com).