Yeast-One Hybrid
Fragments of 60 bp regions of promotors of MtNCED genesMedtr5g025230, Medtr5g025250, Medtr5g02527, predicted to contain
a putative binding site for MtHSFA9(http://plantpan2.itps.ncku.edu.tw)
(Table S1) were cloned into pBait-AbAi vector as bait constructs. The
coding sequence of MtHSFA9 was cloned into the pGADT7-Rec AD
cloning vector as prey construct. The empty pGADT7-Rec plasmid was used
as negative control. Transformation and screening process were carried
out on the media containing SD-Leu and SD-Leu + AbA antibiotic,
respectively, according to the manufacturers’ instructions
(www.clontech.com).