3.2 Identification of differentially expressed proteins and distinct biological processes between GMPP and SMPP
Totally, we identified 130 differentially expressed proteins with 61 up-regulated in GMPP group and 69 up-regulated in SMPP group. Several inflammatory markers such as CRP, SAA1, and SAA2 were found to be up-regulated in SMPP group (Figure 1A). Biological processes such as humoral immune response, acute inflammatory response, protein activation cascade and receptor−mediated endocytosis were enriched in SMPP group (Figure 1B, Table S2). In contrast, enrichment of hydrolase activity, peptidase activity and plasma lipoprotein particles were observed in GMPP group (Figure 1C, Table S3). The KEGG analysis indicated an uncontrolled inflammation catastrophe in SMPP group with the evidence of pathways like complement and coagulation cascades, staphylococcus aureus infection, IL−17 and HIF−1 signaling pathway, and platelet activation were enriched (Figure 1D, Table S4-S5). The complement and coagulation cascades pathway were enriched both in GMPP and SMPP. Complement activation helps recruiting inflammatory and immunocompetent cells and cleaning the extraneous pathogens, and blood coagulation plays a crucial role in the regulation of blood pressure and sodium homeostasis. Figure 1E shows the overview of complement and coagulation cascades pathway alternation between GMPP and SMPP. Many components involving coagulation system including coagulation factors such as F2, F5, F9, F13 and fibrinogen were up-regulated in SMPP group, while complement components C2, C4 and cluster in were altered in GMPP group.