3.2 Identification of differentially expressed proteins and
distinct biological processes between GMPP and SMPP
Totally, we identified 130 differentially expressed proteins with 61
up-regulated in GMPP group and 69 up-regulated in SMPP group. Several
inflammatory markers such as CRP, SAA1, and SAA2 were found to be
up-regulated in SMPP group (Figure 1A). Biological processes such as
humoral immune response, acute inflammatory response, protein activation
cascade and receptor−mediated endocytosis were enriched in SMPP group
(Figure 1B, Table S2). In contrast, enrichment of hydrolase activity,
peptidase activity and plasma lipoprotein particles were observed in
GMPP group (Figure 1C, Table S3). The KEGG analysis indicated an
uncontrolled inflammation catastrophe in SMPP group with the evidence of
pathways like complement and coagulation cascades, staphylococcus aureus
infection, IL−17 and HIF−1 signaling pathway, and platelet activation
were enriched (Figure 1D, Table S4-S5). The complement and coagulation
cascades pathway were enriched both in GMPP and SMPP. Complement
activation helps recruiting inflammatory and immunocompetent cells and
cleaning the extraneous pathogens, and blood coagulation plays a crucial
role in the regulation of blood pressure and
sodium
homeostasis. Figure 1E shows the overview of complement and coagulation
cascades pathway alternation between GMPP and SMPP. Many components
involving coagulation system including coagulation factors such as F2,
F5, F9, F13 and fibrinogen were up-regulated in SMPP group, while
complement components C2, C4 and cluster in were altered in GMPP group.