2.6 Predication of transcription factors (TFs) regulating FCGBP
We used Cistrome Data Browser toolkit
(http://dbtoolkit.cistrome.org/)11to predicate potential TFs that regulating targeted protein FCGBP.
Cistrome Data Browser is a user-friendly web server for querying,
exploring and visualizing ChIP-Seq and chromatin accessibility data.
Since few ChIP-Seq data were publicly available in BEAS-2B cells, an
epithelial cell line isolated from normal human bronchial epithelium, we
query FCGBP’s potential TFs in all available lung cells including A549,
BEAS-2B and NCI-H2052 under the setting of 100 kb distance to
transcription start site in order to include enhancer-type TFs.