2.4 Functional enrichment analysis
The R package “clusterProfiler” 9 was applied for
the enrichment of Gene Ontology (GO) biological process and Kyoto
Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Enrichment
significance was estimated using the default setting and 1000
permutations. Benjamini-Hochberg adjusted P-values less than 0.05 was
considered as statistically significant. The KEGG pathway overview was
implemented by the R package “pathview” 10.