2.7 Connectivity Map Analysis
Drug prediction, mechanism of actions (MoA) and drug-target analyses
were performed on Connectivity Map (CMap) tools
(https://clue.io/) 12.
CMap integrates gene expression signatures, drugs and disease states and
is widely used to predict potential useful drugs, investigate the
internal mechanism and interrelationship of drugs and inform clinical
trials. The query and touchstone functions of CMap website were
performed to drug prediction, MoA and drug-target analysis. Dysregulated
genes between GMPP and SMPP including 47 up-regulated genes and 54
down-regulated genes were subjected to query tool. The “bitr” function
of R package “cluster Profiler” was implemented to convert the uniport
accessions into gene symbols. The R package “Complex Heatmap”13 was used to visualize MoA and drug-target analyses
results. Interaction relationship between the target genes of selected
compounds was analyzed and visualized using STRING
(https://string-db.org/) website.