2.3 Proteomic mass spectrometry
Serum samples from GMPP and SMPP groups were pooled together separately
and subjected to label-free proteomic technology to identify
dysregulated proteins. Maxquant was implemented to process the raw mass
spectrometry data. Proteins with false hits labeled by Maxquant
including “potential contaminant”, “reverse” and “only identified
by site” were excluded. And only proteins detected in both groups were
included in the subsequent analysis. Ultimately, we obtained 276
proteins satisfying above criteria. The LFQ intensity was used to
represent the protein expression level. The raw data are publicly
available on integrated Proteome resources center (iProX) under the
accession number IPX0001738000.