2.4 Statistical analysis
All measurements described were comprised of at least 4 biological
replicates. Data were separately analyzed for variance using SAS/STAT
statistical analysis package (version 6.12, SAS Institute, Cary, NC,
USA). Means were tested by least significant difference at the P 0.05
level (LSD 0.05).
3 | RESULTS
Compared with CK, kernel numbers (KNS) in DS, HS and DHS treatments was
all decreased significantly (Figure 4a). The decrease rate of KNS among
treatments was in the order of
HS<DS<DHS. The KNS was decreased by 27%, 13%, 42%
in DS, HS and DHS treatment, respectively. Furthermore, the subsequent
impacts on grain filling of stress
episodes around flowering was also
observed in both field experiment in Exp. 1
and laboratory incubation
experiment through grain vitro culture in Exp. 2 (Figure 4b, c).
3.1 The response of grain weight to early stress