Materials
Antibodies used in this study were anti-GFP (Clontech), anti-LC3
(Novus), anti-P62 (Proteintech), anti-NCOA4 (Senta Cruz), anti-NDP52
(Novus), anti-NBR1 (Senta Cruz), anti-GAPDH (Proteintech), anti-ub
(Proteintech), anti-VCP (Proteintech), anti-β-Tubulin (Proteintech),
anti-Flag (Senta Cruz). The HTT antibodies 2B7 and the polyQ antibody
MW1 (43) have been described previously. Western-blot was probed with
anti-mouse or anti-rabbit IgG-HRP and visualized using an ECL detection
kit (Millipore).
Chemicals used in this study were gossypol acetate, Rapamycin,
Chloroquine, NMS873, DBEQ. All buffers were prepared in deionized water
and filtered through a 0.2-μm filter.
The primer sequences of plasmids constructs are listed in Table S1 .