Materials
Antibodies used in this study were anti-GFP (Clontech), anti-LC3 (Novus), anti-P62 (Proteintech), anti-NCOA4 (Senta Cruz), anti-NDP52 (Novus), anti-NBR1 (Senta Cruz), anti-GAPDH (Proteintech), anti-ub (Proteintech), anti-VCP (Proteintech), anti-β-Tubulin (Proteintech), anti-Flag (Senta Cruz). The HTT antibodies 2B7 and the polyQ antibody MW1 (43) have been described previously. Western-blot was probed with anti-mouse or anti-rabbit IgG-HRP and visualized using an ECL detection kit (Millipore).
Chemicals used in this study were gossypol acetate, Rapamycin, Chloroquine, NMS873, DBEQ. All buffers were prepared in deionized water and filtered through a 0.2-μm filter.
The primer sequences of plasmids constructs are listed in Table S1 .