Acknowledgements
We thank agencies who provided grant support as well as the many subject families, whose participation makes this research possible. Grant identifications numbers include 5R01HD073434 for WES and 5P01HD035946 for subject recruitment. We give special thanks to Lawrence C. Shimmin and Do-Kyun Kim from the Department of Epidemiology, Human Genetics and Environmental Sciences, School of Public Health at The University of Texas Health Science Center at Houston (UTHealth) for their work and expertise in WES. Special thanks to Karen Soldano and Melanie Garett in Department of Medicine at Duke University for their technical support and providing human neural tubes RNA SAGE information to annotate WES results. LH acknowledges support for attending the International NTD meeting to allow display and discussion of the data with grant number NIH R13 HD 100191.