Anisidine Value
The measurement of anisidine value carried out according to AOCS
Official method Cd, 18-90 (1996). For this purpose, 1g oil sample mixed
with iso-Octane into 25ml conical flasks. Then, 5 ml of this solution
mixed to 1ml of 0.25% p- anisidine solution in glacial acetic acid.
After 10 minutes, solution adsorption read in 350 nm wavelength by
spectrophotometer model UV-6600
(spectroFlex, Germany). The anisidine value was calculated from the
following equation:
\begin{equation}
p-Anisidine\ Value=\frac{25\times(1.2\ As-Ab)}{m}\nonumber \\
\end{equation}As, Ab and m is illustrative of solution adsorption before and after
reaction with p-Anisidine solution, respectively.