Liposome preparation
To prepare liposomes containing green tea extract, in this study, was
applied of mozafari method. The superiority this method than other
methods is the production of liposomes without using any solvent and
detergent (Mozafari & Khosravi-Darani., 2007). In addition to, in this
method do not use of homogenization or sonication methods for reduction
of particle size which cause to destruction of liposome structure. The
green tea extract (0.7-2 %) was first mixed with distilled water. Then
phosphatidylcholine (2.5-4.5 %) and glycerol (3% v/v) were added to
the green tea extract solution. The mixture was agitated on a hot plate
stirrer at temperatures of (50° - 70°C) for different time intervals
(30-60 min) at a mixing speed of 1,000 rpm. Finally, the mixture was
placed at room temperature for one hour in order to increase the
stability of the liposomes. The formulation was kept in a dark bottle at
4°C under nitrogen. Then, physicochemical properties of green tea
liposomes measured such as encapsulation efficiency (EE), particle size
and z- potential and determined optimal conditions to produce to green
tea extract liposomes. In addition to, total polyphenol content of green
tea and free radical scavenging activities of free and liposomal
extract, with using DPPH method measured (Jahanfar et al., 2020).