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Improving glutarate production in Escherichia coli by enhancing the CoA precursors
  • +2
  • Mei Zhao,
  • Yu Zhou,
  • Xue Sui,
  • Guohui Li,
  • Yu Deng
Mei Zhao
Jiangnan University
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Yu Zhou
Jiangnan University
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Xue Sui
Jiangnan University
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Guohui Li
Jiangnan University
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Yu Deng
Jiangnan University
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Peer review status:UNDER REVIEW

21 May 2020Submitted to Biotechnology and Bioengineering
21 May 2020Assigned to Editor
21 May 2020Submission Checks Completed
27 May 2020Review(s) Completed, Editorial Evaluation Pending

Abstract

Glutarate is an attractive C5 platform chemical having wide application in nylon and plasticizer. However, microbial glutarate is mainly accumulated in the degradation of lysine and other methods were rarely explored for producing the glutarate from glucose directly. Here, we utilized a reversed adipic acid degradation pathway (RADP) and improved the glutarate production by increasing the precursors (malonyl-CoA and acetyl-CoA) based on the previously studied strain Bgl4146. Specifically, the conversion system of intracellular acetyl-CoA to malonyl-CoA was firstly constructed and optimized to balance acetyl-CoA synthase and acetyl-CoA carboxylase under different dissolved oxygen, enhancing the glutarate production from 0.09 g/L to 0.49 g/L. Then, modulating CoA balance by monitoring the expression of acetate kinase and pyruvate dehydrogenase resulting in a rise in glutarate titer up to 0.70 g/L. Finally, the optimized strain Bgl51464 was able to produce 7.97 g/L glutarate in a 5-L bioreactor. This strategy was described here, which could lay a certain foundation for the development of effective CoA balance to produce industrially high value-added chemicals.