2.4 Barcoding analysis based on COI
Bidirectional sequencing was
employed to guarantee the precision of sequencing. We aligned the COI
gene using the ClustalW program from MEGA 7.0 (Tamura et al., 2013)
package for each order with default parameters (Zhou et al., 2010). The
amino acid translation was
conducted
to ensure that no gaps or stop codons existed in the alignment. Totally,
we obtained 1227 COI sequences from 189 species. Detailed information
(locality data, habitat, altitude, collector, identifier, taxonomic
classifications, habitus images and DNA barcode sequences etc.) for each
voucher specimen was deposited to the Barcode of Life Data System (BOLD)
at http://www.boldsystems.org (Ball et al., 2007), under
the dataset project “XJDQD” (Process IDs: XJDQD001-18-XJDQD1275-18).