2.4.1 Phylogenetic tree
Neighbor-Joining (NJ) method in MEGA 7.0 software was used for tree
construction (Satiou et al., 1987) with
Kimura-2-Parameter (K-2-P)
genetic distances (Kimura et al., 1980). Meanwhile, to evaluate the
accuracy of DNA barcoding for classifying species as well as the
evolutionary relationship of species, we downloaded the sequencing data
of relevant species from BOLD, including those from Germany, United
States, Mexico, Canada, Norway, Italy, Finland, Japan, Iran, etc.
(Raupach et al., 2014; Stein et al., 2013) (Part I, Supporting
information). A total of 1000 replicates were utilized to detect the
reliability of each branch of the tree in order to obtain bootstrap
values.