3.5 BIN analysis
In the present study, 1227 records generated 201 different BINs. In
particular, 117 BINs were not recorded previously in the BOLD database
and most sequences representing new endemic species received new BIN
assignments. The Diptera speciesChironomus sp. XJ andC. heterodentatus shared the same BIN [AAW4009], P.
bullum shared a BIN assignment
[ACB4789] with P. laetum. BIN Discordance analysis was
performed on BOLD (February 2018), and the results showed that two BINs
were discordant with our prior taxonomic assignments, which was
attributed to haplotype sharing and low interspecific divergence. A
number of 141 BIN clusters were
found to be taxonomically concordant with other barcode data on BOLD,
that is, they were assigned to the same species. A number of 58 records
are singletons, implying that these BINs were represented by only one
sequence. Nine species were assigned to a total of
21 BINs (Table S2).