2.4.2 Barcode Gap Analysis and BIN analysis
“Barcode Gap Analysis” tool in
the BOLD system was carried out to compare the distribution of distances
within each species and the distance to the nearest neighbor of each
species. Species were tested for the
presence of the Barcode Gap between
maximum intraspecific genetic distance and minimum interspecific
distance according to Ratnasingham & Hebert (2007). Within the BOLD,
the BIN system group sequence data into clusters of closely COI barcode
sequences that are assigned to a globally unique identifier, termed BIN
(Barcode Index Number) (Ratnasingham & Hebert, 2013). BINs for species
clusters also enable the delineation of geographical clades, which might
reflect local environmental features (Jerome et al., 2017).