2.8 Functional annotation of protein-coding genes.
Predicted genes were subjected to functional annotation by performing a BLAST v2.2.3 homologue search against the final gene set (Altschul et al. 1990). BLASP (Altschul et al. 1990) was used to predict gene function through searches against five databases (E-value=1e-5), including SwissProt (Boeckmann et al., 2003), TrEMBL(Boeckmann et al., 2003), KEGG (Kanehisa et al., 2000), InterPro (Zdobnov et al., 2001), Nr(Sujana et al., 2005), Swissprot(Bairoch and Apweiler, 2000), KOG (koonin et al., 2004), and GO (Ashburner et al., 2000).