2.2 Particle characteristics of these Cap-3M2e
VLPs
These Cap-3M2e proteins were dialyzed into the assembly buffer (10 mM
Tris-HCl, 100 mM NaCl (pH 8.0)) and formed VLPs. The shape, size, size
distribution and zeta potential of these Cap-3M2e VLPs were
characterized by transmission electron microscopy (TEM) (JEM-1400; JEOL,
Tokyo, Japan) and dynamic light scattering (DLS) (Malvern,
Worcestershire, UK).