Immunohistochemistry : Rabbit anti 5-HT and Mouse anti 5-HT Double Labeling
The same paraffin sections and the same staining protocol as mentioned above were applied for 5-HT double labelling to investigate co-localization of two 5-HT markers. Primary antibodies were rabbit anti human 5-HT (ImmunoStar, Cat # 20080, Lot # 1431001, 1:2000) and mouse anti-5-HT (ThermoFisher Scientific, Cat # MA5-12111, 1:100); secondary antibodies were Alexa 488 goat anti-rabbit IgG (Jackson ImmunoResearch, 1:300) for rabbit 5-HT staining and Cy3 conjugated goat anti-mouse IgG (Jackson ImmunoResearch, 1:200) for mouse 5-HT staining.