Immunohistochemistry : Mouse anti 5-HT with Protease XXV for
Enzyme-Induced Epitope Retrieval and Heat-Induced Epitope Retrieval with
citrate buffer
Adjacent thin sections of 2 µm were studied for mouse anti 5-HT staining
with two different antigen retrieval techniques. Enzyme-Induced epitope
retrieval with protease XXV was recommended by the manufacturer but we
also performed heat-induced epitope retrieval to be able to double label
with anti 5-HT4R. We added this test to observe whether
the two techniques produced the same staining results.
All immunostaining was examined with a Leica DMRXA2 microscope with x20
objective lens. All pictures were taken using a Retiga Imaging digital
camera attached to the microscope and an Apple computer with Volocity
software (Improvision Inc., Montreal, QC). For each colon biopsy
section, six to eight areas covering the whole section were selected.