Immunohistochemistry : Mouse anti 5-HT with Protease XXV for Enzyme-Induced Epitope Retrieval and Heat-Induced Epitope Retrieval with citrate buffer
Adjacent thin sections of 2 µm were studied for mouse anti 5-HT staining with two different antigen retrieval techniques. Enzyme-Induced epitope retrieval with protease XXV was recommended by the manufacturer but we also performed heat-induced epitope retrieval to be able to double label with anti 5-HT4R. We added this test to observe whether the two techniques produced the same staining results.
All immunostaining was examined with a Leica DMRXA2 microscope with x20 objective lens. All pictures were taken using a Retiga Imaging digital camera attached to the microscope and an Apple computer with Volocity software (Improvision Inc., Montreal, QC). For each colon biopsy section, six to eight areas covering the whole section were selected.