Hardware

Results

Designs to detect AMR

1.      Resistance to quinolones:  AMR to quinolones have been reported due to mutations in the DNA gyrase  genes (gyrA and gyrB) and DNA topoisomersae IV (parC and parC). Point mutation in the 83SertoPhe in gyrA is the most commonly reported . In this study we demonstrate that our design . Cas12b has been demonstrated to be less sensitive to single mismatch between the gRNA and the target strand. In order to detect the SNP in 83Ser, we introduce a mismatch in the gRNA as shown in Figure X. Additionally, the target region does not include a PAM sequence which is introduced using the site-directed mutagensis in the forward primer (). The reverse primer  X amplies a region of Xbp.
2. Resistance to beta-lactmases: Akinyemi et al., (2015) reveal in their study the emergence of beta lactamase producing gene blaCTX-M-1 S. typhi strain in lagos, Nigeria as an agent of persistent pyrexia.