CRISPR Scissors for molecular detection
PCR and real-time PCR has been the 'gold standard' method for diagnostics due to his high sensitivity and specificity. However, they depend on thermocycler which could be expensive and also slow . The discovery of isothermal methods of amplifying the nucleic acid target in the last two decades has revolutionized the POC diagnostics by reducing the amplification time and bypassing the need for expensive thermocycler. Recombinase polymerase amplification (RPA) \cite{Piepenburg_2006} and LAMP \cite{Notomi2000} are the two most popular PCR alternatives that has seen a wide spread application in rapid-low-cost POC diagnostics. LAMP relies on a single polymerase and a complex(I am looking for another word) primer design for amplification at 65C. RPA depends on polymerase, recombinase, single strand binding protein for amplification at 37C. The model Assisted by a loading factor, recombinase binds to primers to form a primer-protein complex, which then binds to the dsDNA at the primer binding region. A single strand binding protein stabilizes the