Ilustration of one-pot Cas12b assay to diagnose enteric fever. In the first step, in-house produced RPA enzymes, Bsu, UvsY, UvsX and GP32 combined with Cas12b, ssDNA reporter, gRNA, primers, S. Typhi/S. Paratyphi template and other components of RPA assay (see Methods), and incubated at 37C for 30 min for target amplification. The mixture is next incubated at 55 C for the activation of Cas12b , which upon recognition of the PAM sequence, binds to the target strand and initiates non-specific cleavage of the fluorescent-quencher tagged ssDNA. The resulting fluorescence is then measured using a home built detector.