Add WHO guidelines 

CRISPR Scissors for molecular detection

PCR has been the 'gold standard' for diagnostics due to his high sensitivity and specificity. However, using PCR depends on thermocycler which could be expensive and also slow .The discovery of isothermal methods of amplifying the nucleic acid target in the last two decades has revolutionized the POC diagnostics by reducing the amplification time and bypassing the need for expensive thermocycler. Recombinase polymerase amplification (RPA) \cite{Piepenburg_2006} and LAMP \cite{Notomi2000} are the two most popular PCR alternatives that could be applied for low cost POC diagnostics in LMICs.  LAMP relies on a single polymerase and a complex primer design for amplification at 65C. RPA depends on polymerase, recombinase, single strand binding protein for amplification at  37C.