Genetic clustering
The assignment test analysis using STRUCTURE also showed evidence of
nuclear introgression in three plots (Fig. 4). As the species were
identified, the most appropriate K was K = 2 for all plots, indicating
that the colonies of both Nanjing and Wuhan were appropriately divided
into two species-specific genotypic clusters, although a few gene
introgressions were observed. In contrast, the populations of R.
flaviceps and R. chinensis in Chengdu were much more mixed and
showed little evidence of species differentiation.