Airway Diseases |
Platform |
Sample Source |
Main findings |
Reference |
AR |
Microarray |
Human nasal mucosa |
Described the gene expression
profiles of CD4 T cells and CD45+HLA-DR+ cells in patients with
experimental allergic rhinitis |
[68] |
|
NovaSeq |
PBMCs |
Single-cell immunoassays after immunotherapy for
allergic rhinitis revealed functional inhibition and clonal
transformation of pathogenic Th2 cells |
[8] |
CRS |
10X |
Nasal irrigation solution |
Cellular stress responses
indicated by increased MTRNR2L12, MTRNR2L8 and NEAT1 expression in
immune cell subpopulations |
[69] |
|
10X |
Human nasal polyps |
GATA3 expression is a feature of polyp Treg
cells, whereas Th2 cells highly express TCN1, CD200R, HPGDS and are
enriched in genes involved in lipid metabolism |
[70] |
Asthma |
10X |
Mouse lung tissue |
MiR-155 deficiency altered lung
immune cell heterogeneity in a mouse model of severe asthma |
[71] |
|
10x |
Monkey bronchial epithelium and lung tissue |
An analysis of
intercellular interactions revealed a complex network among stromal
cells, MCs, and macrophages |
[72] |
COPD |
Illumina NextSeq 500 |
Human alveolar lavage fluid |
Coexistence
of monocyte-derived macrophages with classical alveolar macrophage AMs
in the airways of healthy subjects and COPD patients |
[73] |
|
10X |
Human lung tissue |
IGFBP5 and QKI as Identification Factors for
ciliated epithelial cell genes associated with severe COPD |
[33] |
|
Drop-seq |
Human small airway epithelium |
Identified novel
cell-specific expression of lung disease-associated genes in human small
airway epithelium |
(GSE173896) |
IPF |
10X |
Human lung tissue |
Provided a single-cell atlas of the IPF
lung; identified rare abnormal basal-like cells, as well as two separate
fibrosis prototypes in stromal cells associated with stromal populations |
[38] |
|
10X |
Mouse lung tissue fragments |
Reports on the development of a
new computational method for assessing differences in cell-cell
interactions between conditions by using single-cell RNA sequencing data |
[74] |
|
10X |
Human lung tissue |
Describes the proliferation of
SPP1/MERTK-expressing macrophages in idiopathic pulmonary fibrosis |
[75] |
|
Illumina NextSeq 500 |
Human lung tissue |
Shows that NOTCH2 restricts
the differentiation of basal cells to ciliated cells and demonstrates
that NOTCH3 inhibits secretory differentiation |
[37] |
COVID-19 |
Drop-seq |
Human lung epithelial cells |
Uses airway
epithelial cells to show that ACE2 is an in vitro extension of
the human interferon-stimulated gene (ISG) to viral infections in
vivo
|
[76] |
|
inDrops |
Mouse bronchial epithelium |
Single-cell mapping of airway
epithelium revealed CFTR-rich lung ionocytes |
[77] |
|
10X |
Human lung epithelial cells |
An immunophenotypic analysis of
COVID-19 and influenza highlighted the type I IFN response in severe |
[78] |
NPC |
Illumina NovaSeq 6000 |
Human nasopharyngeal carcinoma tissue |
Highly representative features in TME were reported, including
phenotypic abundance, genetic alterations, immune dynamics that
profoundly affect patient prognosis and treatment outcomes, clonal
amplification, developmental trajectories, and molecular interactions |
[51] |