Airway Diseases Platform Sample Source Main findings Reference
AR Microarray Human nasal mucosa Described the gene expression profiles of CD4 T cells and CD45+HLA-DR+ cells in patients with experimental allergic rhinitis [68]
NovaSeq PBMCs Single-cell immunoassays after immunotherapy for allergic rhinitis revealed functional inhibition and clonal transformation of pathogenic Th2 cells [8]
CRS 10X Nasal irrigation solution Cellular stress responses indicated by increased MTRNR2L12, MTRNR2L8 and NEAT1 expression in immune cell subpopulations [69]
10X Human nasal polyps GATA3 expression is a feature of polyp Treg cells, whereas Th2 cells highly express TCN1, CD200R, HPGDS and are enriched in genes involved in lipid metabolism [70]
Asthma 10X Mouse lung tissue MiR-155 deficiency altered lung immune cell heterogeneity in a mouse model of severe asthma [71]
10x Monkey bronchial epithelium and lung tissue An analysis of intercellular interactions revealed a complex network among stromal cells, MCs, and macrophages [72]
COPD Illumina NextSeq 500 Human alveolar lavage fluid Coexistence of monocyte-derived macrophages with classical alveolar macrophage AMs in the airways of healthy subjects and COPD patients [73]
10X Human lung tissue IGFBP5 and QKI as Identification Factors for ciliated epithelial cell genes associated with severe COPD [33]
Drop-seq Human small airway epithelium Identified novel cell-specific expression of lung disease-associated genes in human small airway epithelium (GSE173896)
IPF 10X Human lung tissue Provided a single-cell atlas of the IPF lung; identified rare abnormal basal-like cells, as well as two separate fibrosis prototypes in stromal cells associated with stromal populations [38]
10X Mouse lung tissue fragments Reports on the development of a new computational method for assessing differences in cell-cell interactions between conditions by using single-cell RNA sequencing data [74]
10X Human lung tissue Describes the proliferation of SPP1/MERTK-expressing macrophages in idiopathic pulmonary fibrosis [75]
Illumina NextSeq 500 Human lung tissue Shows that NOTCH2 restricts the differentiation of basal cells to ciliated cells and demonstrates that NOTCH3 inhibits secretory differentiation [37]
COVID-19 Drop-seq Human lung epithelial cells Uses airway epithelial cells to show that ACE2 is an in vitro extension of the human interferon-stimulated gene (ISG) to viral infections in vivo [76]
inDrops Mouse bronchial epithelium Single-cell mapping of airway epithelium revealed CFTR-rich lung ionocytes [77]
10X Human lung epithelial cells An immunophenotypic analysis of COVID-19 and influenza highlighted the type I IFN response in severe [78]
NPC Illumina NovaSeq 6000 Human nasopharyngeal carcinoma tissue Highly representative features in TME were reported, including phenotypic abundance, genetic alterations, immune dynamics that profoundly affect patient prognosis and treatment outcomes, clonal amplification, developmental trajectories, and molecular interactions [51]