Methodology
For the analysis, the SNV (Single-Nucleotide Variant) files were
examined from the data package. The data files contained information
which provided the locus of each mutation, the zygosity, variation type,
and the gene on which it occurred. The ‘Match’ column was not present
within the data and was created with a formula as a detection method for
any coding mutations. The formula was an IF function which compared each
cell from the reference sequence with the corresponding cell from the
allele 2 sequence. Whenever there was a mismatch, the function would
automatically flag each mutation with an ‘X’ symbol in the corresponding
cell within the ‘Match’ column. After the IF function was imported into
the ‘Match’ column, it was applied to the entire genome to search for
any mutations. Once the comparison was complete, the ‘Match’ column was
filtered out to only show the base pair mismatches (occurred only when
an ‘X’ was present in the ‘Match’ column) [Figure 1]. These
procedures were followed for both the primary (PUM) and metastases (MUM)
files.