Figure legends.
Figure 1. Evaluation of bacterial load in the prescapular LN of HF and Zebu cattle.
HF ( and ) and Zebu ( and ) cattle were vaccinated ( and ) or not ( and ) as described in figure 1 and in materials and methods. Prescapular lymph nodes were harvested at post-mortem, three weeks post-intranodal challenge. Organs were macerated and an aliquot of the tissue macerate was plated in 7H11 plates. Counts are presented as cfu/organ; the limit of detection for this assay is 50 cfu.
Figure 2. Longitudinal immune responses in Zebu and HF cattle to mycobacterial antigens. Immune responses were evaluated as the secretion of IFNγ by peripheral blood cells from HF (A) and Zebu (B) cattle that had been vaccinated (white and horizontally hashed bars) with c106 BCG SSI cfu subcutaneously or not (black and diagonally hashed bars). Cells were stimulated with PPD-A or PPD-B. Vaccination occurred at week 0 and all animals were challenged in both prescapular lymph nodes with c 107 BCG Tokyo cfu each lymph node at week 8 (arrow). For a closer description of statistics, please see text in results and discussion.
Supplementary figure 1.
Longitudinal immune responses in Zebu and HF cattle to PPD-A. Immune responses were evaluated as the secretion of IFNγ by peripheral blood cells stimulated with PPD-A from HF (black and horizontally hashed bars) and Zebu (white and diagonally hashed bars) cattle that had been vaccinated (black and white bars) with c 106BCG SSI cfu subcutaneously or not (horizontally and diagonally hashed bars). Vaccination occurred at week 0 and all animals were challenged in both prescapular lymph nodes with c 107 BCG Tokyo cfu each lymph node at week 8 (arrow). For a closer description of statistics, please see text in results and discussion.