Evaluation of immune responses.
Immune responses were evaluated as production of interferon gamma (IFNγ)
in supernatants of peripheral blood cells incubated overnight at
37oC in a 5% CO2 and 95% humidity
atmosphere with purified protein derivative from Mycobacterium
avium (PPD-A) or M. bovis (PPD-B), or medium alone as negative
control (Villarreal-Ramos et al., 2014). Levels of IFNγ were determined
by using the Bovigam™ assay (Prionics). Data are shown as mean ± SEM.