Cattle
Thirty-two Zebu cattle and 31 Holstein-Friesian (HF) cattle were sourced from farms free of TB. Due to their nature, Zebu cattle were sourced from the southern subtropical regions of Mexico (State of Veracruz) and Holstein-Friesian cattle were sourced from the central region of Mexico (State of Mexico). Zebu cattle varied between 2 and 8 months of age with a median age of 3 months, whilst HF cattle varied between 4 and 7 months of age with a median age of 6 months.