Gene expression analysis
Expression analysis was performed as described in Samarth et al .
(2019). The primer sequences used are listed in Table S2. In addition toCpFT1, CpFT2, CpFT3, CpFT4, CpFT5 and CpTFL1 , homologues
of other floral-promoting genes known to regulate the expression ofFT- like genes (Song et al., 2013) including CpGI ,CpHd1 , CpEhd3 , CpMADS1 , CpTPS1, CpMADS50 and
a temperature regulator of flowering in temperate grasses
(CpVRN1 ) were also included in the expression studies. Despite
much effort, the expression of CpFT1 could not be detected in the
leaf samples.