Phylogenetic, structural and functional analysis of the
PEBP-sequences in C. pallens
The reference ‘C. pallens’ transcriptome assembly
published in Samarth et al. (2019) was used to identify the potential
homologues of the PEBP protein family (Table 2). The identified
homologous PEBP sequences were further clustered into their respective
sub-families using a phylogenetic approach. The PEBP-phylogenetic tree
consisting of 49 protein sequences (Table S1) was reconstructed using
the maximum likelihood method with Jukes Cantor substitution matrix and
1000 bootstrap replication using the PhyML package. Homologous PEBP
protein sequences were also modelled using a homology modelling approach
at the SWISS-PROT server and visualised using Pymol (student version).
For functional analysis, homologous PEBP-gene sequences (CpFT1,
CpFT2, CpFT3, CpFT4, CpFT5 and CpTFL1 ) were transformed intoArabidopsis thaliana ecotype Landsberg erecta ft-1 mutant
plants using the pB2GW7 vector. Wild-type Landsberg plants flower after
producing ~ 8-10 leaves whereas ft-1 is a
late-flowering mutant, flowering after ~20 leaves
(Martinezzapater & Somerville, 1990).