Fig. 3: (a) Heat-map of 50 most significantly differentially expressed genes in the leaves of 17Control and 17Hot transplants (17H)collected during the inductive summer period (January-2017). The heatmap was constructed using the R packages DESeq2 and heatmap.2 . Hierarchical clustering is based on the Spearman’s coefficient calculated from the log-transformed RNA-seq data. (b) PCA plotof the transcriptomic data from three separate biological replicates, each for 17 control and 17Hot transplants. (c) Differentially expressed flowering-pathway genes in C. pallens. Logarithmic (log2) fold change in the orthologous floral genes found to be significantly differentially expressed in the leaves of the 17Hot transplants that flowered in the next season relative to the leaf samples from the control plot plants that remained vegetative. (d)Gene Ontology analysis. GO classification of the upregulated genes (left-hand side) and downregulated genes (right-hand side) in the leaves of the 17Hot transplants that flowered compared to the leaves from the plants at the control plot with a significance level of 0.05 after the Benjamini and Hochberg FDR correlation. (BP: Biological Process, CC: Cellular Component, MF: Molecular Functions)