Phylogenetic, structural and functional analysis of the PEBP-sequences in C. pallens
The reference ‘C. pallens’ transcriptome assembly published in Samarth et al. (2019) was used to identify the potential homologues of the PEBP protein family (Table 2). The identified homologous PEBP sequences were further clustered into their respective sub-families using a phylogenetic approach. The PEBP-phylogenetic tree consisting of 49 protein sequences (Table S1) was reconstructed using the maximum likelihood method with Jukes Cantor substitution matrix and 1000 bootstrap replication using the PhyML package. Homologous PEBP protein sequences were also modelled using a homology modelling approach at the SWISS-PROT server and visualised using Pymol (student version). For functional analysis, homologous PEBP-gene sequences (CpFT1, CpFT2, CpFT3, CpFT4, CpFT5 and CpTFL1 ) were transformed intoArabidopsis thaliana ecotype Landsberg erecta ft-1 mutant plants using the pB2GW7 vector. Wild-type Landsberg plants flower after producing ~ 8-10 leaves whereas ft-1 is a late-flowering mutant, flowering after ~20 leaves (Martinezzapater & Somerville, 1990).