Fig. 2: Gene expression analysis (relative to reference genes)
of the orthologous PEBP-sequences in C. pallens during the
inductive summer period in plants from various sites and fates [either
remaining vegetative (veg) or flowering (flw) the next season]. (a)
The x-axis indicates the site from where leaf samples were taken:
16Control and 17Control, control plants at 1070 m in 2016 and 2017;
16Hot and 17Hot, plants translocated from 1070 m to 15 m at University
of Canterbury sampled in 2016 and 2017; 16Cool, plants transplanted in
2015 from 1070 m to 1520 m Mt Hutt sampled in 2016 that flowered in
2017. (b) Expression of CpATFL1 in the leaves of tillers that
either flowered or remained vegetative in four separate site/year
combinations: from left, University of Canterbury (UC, 15 m, 16Hot),
1520 m Mt Hutt (16Cool), UC (17Hot), and 1070 m Mt Hutt (18Control). The
data are represented by the mean ± S.D. of two biological replicates
each with three technical replicates. The data were calculated using
relative fold changes against two internal reference genes, CpExPand CpTHP . (two-way ANOVA, ** P < 0.01, * P <
0.05; Table S5).