Cytokine assays
Cytokine concentrations in the pancreas were measured by the
enzyme-linked immunosorbent assay (ELISA), as previously described
[24, 25]. Concentrations of IFN-α, IFN-γ, IL-6, IL-13, IL-33, and
TNF-α were determined using ELISA kits obtained from R&D Systems
(Minneapolis, MN) and eBioscience.