Histological analysis
The mice were sacrificed 3 hours after the final injection of poly (I:C)
or oral administration of K. pneumoniae . Pancreatic samples were
subjected to fixation with 10% formalin followed by hematoxylin and
eosin (H&E) staining; subsequently, pathological assessment was done by
using a scoring system for AIP in accordance with previous reports
[14, 16, 20]. Pancreas inflammation was scored as follows: 0,
pancreas without mononuclear cell infiltration; 1, mononuclear cell
aggregation and/or infiltration within the interstitium without
parenchymal destruction; 2, focal parenchymal destruction with
mononuclear cell infiltration; 3, diffuse parenchymal destruction but
with retention of some intact parenchymal areas; 4, destruction of
almost all pancreatic tissue, except for the pancreatic islets, or its
replacement with fibrosis or adipose tissue [14, 16, 20]. At least
two histological microscopy images (Biozero, BZ-8100, Keyence) were
captured from each slide.