Figure 3. Expression of α5 subunit-containing GABAARs in CA1 . (A-D) Confocal microscopy Z-stacks at 63× magnification showing α5 subunit-containing GABAAR expression on pyramidal neurons (CaMKII- α, green, FITC), CR interneurons (green, Alexa 488), SST interneurons (red, Texas Red), and CCK interneurons (red, Texas Red) in wild-type(i) and AppNL-F/NL-F animals(ii) . Panels show individual channels and merged image with the nuclear stain DAPI (blue). Representative cells are outlined with white circles. (E) Representative image taken at 20× magnification to exemplify the region of data acquisition. Layers are labelled: alveus (A), stratum oriens (SO), stratum pyramidale (SP), stratum radiatum (SR), stratum lacunosum moleculare (SLM). The image is labelled with the cell names in the locations they were imaged. (F) Analysis of α5 subunit-containing GABAAR expression on the soma of the four types of cells investigated. (G) Analysis of α5 subunit-containing GABAAR signal on the dendrites of CR cells, SST cells and pyramidal neurons (F-G) Results are expressed as a scatter plot ± standard error of the mean (results not significant, P > 0.05), of Pearson correlation coefficient as a measure of colocalisation, after application of Fisher’s transformation.