Figure 3. Expression of α5 subunit-containing
GABAARs in CA1 . (A-D) Confocal microscopy
Z-stacks at 63× magnification showing α5 subunit-containing
GABAAR expression on pyramidal neurons (CaMKII- α,
green, FITC), CR interneurons (green, Alexa 488), SST interneurons (red,
Texas Red), and CCK interneurons (red, Texas Red) in wild-type(i) and AppNL-F/NL-F animals(ii) . Panels show individual channels and merged image with the
nuclear stain DAPI (blue). Representative cells are outlined with white
circles. (E) Representative image taken at 20× magnification to
exemplify the region of data acquisition. Layers are labelled: alveus
(A), stratum oriens (SO), stratum pyramidale (SP), stratum radiatum
(SR), stratum lacunosum moleculare (SLM). The image is labelled with the
cell names in the locations they were imaged. (F) Analysis of
α5 subunit-containing GABAAR expression on the soma of
the four types of cells investigated. (G) Analysis of α5
subunit-containing GABAAR signal on the dendrites of CR
cells, SST cells and pyramidal neurons (F-G) Results are
expressed as a scatter plot ± standard error of the mean (results not
significant, P > 0.05), of Pearson correlation
coefficient as a measure of colocalisation, after application of
Fisher’s transformation.