2.3 Analytical methods
Concentrations of cell, glycerol, methanol, reactive oxygen species
(ROS), and oxygen uptake rate (OUR), specific glycerol/methanol rate
were
determined
by the reported method.10-12 Cell viability was
measured by fluorescein isothiocyanate (FITC) labeling
technique.13 The activities of alcohol oxidase (AOX),
formaldehyde dehydrogenase (FLD), formate dehydrogenase (FDH), hLYZ and
SDS-PAGE analysis were measured or analyzed using the same procedures
described in our previous reports.9,10