Figure Captions
Figure 1 Curves of fermentation data during the glycerol feeding phase when using different glycerol feeding strategies.
A, D & G: “high DO-low glycerol concentration” control strategy; B, E & H: “low DO-high glycerol concentration” control strategy; C, F & I: periodic DO control strategy. ━ : DO; ■: glycerol concentration; ●: cell concentration; ▲: ROS concentration; ♦: cell mortality.
Figure 2 Glycerol metabolism patterns when using different glycerol feeding strategies.
■: “high DO-low glycerol concentration” control strategy; ▲: “low DO-high glycerol concentration” control strategy; ●: periodic DO control strategy.
Figure 3 Variations of cell concentration, hLYZ activities and SDS-PAGE analysis results of hLYZ production during the methanol induction phase when using different glycerol feeding strategies.
■/strategy A: “high DO-low glycerol concentration” control strategy; ▲/strategy B: “low DO-high glycerol concentration” control strategy; ●/strategy C: periodic DO control strategy.
Figure 4 Variations of major fermentation/induction data of hLYZ production during the methanol induction phase when using different glycerol feeding strategies.
(I) Variations of DO and OUR. A: “high DO-low glycerol concentration” control strategy; B: periodic DO control strategy. ━ : DO; …: OUR.
(II) Variations of specific activities of AOX, FLD, FDH and specific methanol consumption rate. ■: “high DO-low glycerol concentration” control strategy; ●: periodic DO control strategy.