2.3 Analytical methods
Concentrations of cell, glycerol, methanol, reactive oxygen species (ROS), and oxygen uptake rate (OUR), specific glycerol/methanol rate were determined by the reported method.10-12 Cell viability was measured by fluorescein isothiocyanate (FITC) labeling technique.13 The activities of alcohol oxidase (AOX), formaldehyde dehydrogenase (FLD), formate dehydrogenase (FDH), hLYZ and SDS-PAGE analysis were measured or analyzed using the same procedures described in our previous reports.9,10