Figure Captions
Figure 1 Curves of fermentation data during the glycerol
feeding phase when using different glycerol feeding strategies.
A, D & G: “high DO-low glycerol concentration” control strategy; B, E
& H: “low DO-high glycerol concentration” control strategy; C, F &
I: periodic DO control strategy. ━ : DO; ■: glycerol concentration; ●:
cell concentration; ▲: ROS concentration; ♦: cell mortality.
Figure 2 Glycerol metabolism patterns when using different
glycerol feeding strategies.
■: “high DO-low glycerol concentration” control strategy; ▲: “low
DO-high glycerol concentration” control strategy; ●: periodic DO
control strategy.
Figure 3 Variations of cell concentration, hLYZ activities and
SDS-PAGE analysis results of hLYZ production during the methanol
induction phase when using different glycerol feeding strategies.
■/strategy A: “high DO-low glycerol concentration” control strategy;
▲/strategy B: “low DO-high glycerol concentration” control strategy;
●/strategy C: periodic DO control strategy.
Figure 4 Variations of major fermentation/induction data of
hLYZ production during the methanol induction phase when using different
glycerol feeding strategies.
(I) Variations of DO and OUR. A: “high DO-low glycerol concentration”
control strategy; B: periodic DO control strategy. ━ : DO; …:
OUR.
(II) Variations of specific activities of AOX, FLD, FDH and specific
methanol consumption rate. ■: “high DO-low glycerol concentration”
control strategy; ●: periodic DO control strategy.