Figure 4
In a summary, a novel periodic DO control strategy for efficient hLYZ production was proposed. With this novel control strategy: a higher cell concentration (~120 g-DCW/L) with healthy and functional skeleton was achieved at the end of cell growth phase by controlling ROS concentration below 48.35 Fluorescence intensity g-DCW-1; (2) P. pastoris metabolic activities in the successive methanol induction phase were largely enhanced, leading to an efficient hLYZ production when the same methanol induction strategy was applied.