Figure 4
In a summary, a novel periodic DO control strategy for efficient hLYZ
production was proposed. With this novel control strategy: a higher cell
concentration (~120 g-DCW/L) with healthy and functional
skeleton was achieved at the end of cell growth phase by controlling ROS
concentration below 48.35 Fluorescence intensity
g-DCW-1; (2) P. pastoris metabolic activities
in the successive methanol induction phase were largely enhanced,
leading to an efficient hLYZ production when the same methanol induction
strategy was applied.