RNA isolation
RNA samples were obtained from the biobank of the A.C. Camargo Cancer Center and GRAACC; samples were extracted from tissues using the QIASymphony DNA Mini kit (QIAGEN ) and the RNeasy Mini Kit (QIAGEN ), according to manufacturer’s recommendations. The quality of RNA samples was verified by microfluidics-based electrophoresis (Bioanalyzer, Agilent Technologies; CA, USA), and only RNA samples with RNA Integrity Number (RIN) >7.0 were used.