Laboratory Testing
Infants with findings suggestive of congenital infections were submitted to testing for syphilis (treponemal and reaginic tests) and toxoplasmosis (IgM and IgG ELISA). Also, blood, saliva, urine and/or liquor were tested for enterovirus, parvovirus, HHV-6, HSV-I, HSV-II, and CMV via PCR assays. Infants with CNS abnormalities for whom a blood sample had been obtained within three months of age were tested for anti-IgM ZIKV antibodies (ZIKV DetectTM 2.0 IgM Capture Elisa, InBios International, Inc. Seattle, WA, USA), and ZIKV-RNA (CDC Trioplex Real-time RT-PCR Assay).