Influence of Epidermal Growth Factor (EGF) on wound healing
To determine the role of the EGF pathway on wound healing, EGF (25ng/ml, StemCell) 13 was added to the basolateral media 7 days prior to wounding and replenished every two days with media changes. In separate experiments EGF was neutralized by adding the specific EGF receptor blocker, Erlotinib HCl (2µg/ml) (OSI-744, Shelleckchem)14, to the basolateral media 7 days prior to wounding. Media with inhibitor was replenished every two days.