Influence of IL-13 on wound healing
To determine the role of IL-13 on wound healing, exogenous IL-13 (10ng/ml) 12 was added to the basolateral media 7 days prior to wounding and replenished every two days with media changes. In separate cultures IL-13 was neutralized by adding human IL-13 Mab (Clone 31606, R & D Systems) to the basolateral media 7 days prior to wounding and replenished every two days when media was changed.