Influence of Epidermal Growth Factor (EGF) on wound
healing
To determine the role of the EGF pathway on wound healing, EGF (25ng/ml,
StemCell) 13 was added to the basolateral media 7 days
prior to wounding and replenished every two days with media changes. In
separate experiments EGF was neutralized by adding the specific EGF
receptor blocker, Erlotinib HCl (2µg/ml) (OSI-744, Shelleckchem)14, to the basolateral media 7 days prior to wounding.
Media with inhibitor was replenished every two days.