Cytokine assays
The apical surface of ALI cultures was washed with 100µl PBS and
analysed for secretion of Interleukin (IL)-13, Interferon beta (INFβ),
IL-1β, IL-33 and Transforming growth factor beta (TGFβ), using the
Luminex Assay (Millipore) or AlphaLISA kits (Perkin Elmer) following
manufacturer’s recommendations. Limits of detection for the different
assays are shown in Table E1.