Cytokine assays
The apical surface of ALI cultures was washed with 100µl PBS and analysed for secretion of Interleukin (IL)-13, Interferon beta (INFβ), IL-1β, IL-33 and Transforming growth factor beta (TGFβ), using the Luminex Assay (Millipore) or AlphaLISA kits (Perkin Elmer) following manufacturer’s recommendations. Limits of detection for the different assays are shown in Table E1.