Statistical analysis
Data were generally not normally distributed. Comparisons between groups were undertaken using Kruskal-Wallis, Mann-Whitney, Fishers-Exact Test, Analysis of Variance on Ranks, and Repeated Measures Analysis of Variance on Ranks as appropriate using GraphPad Prism 7.0 (GraphPad Inc., La Jolla, CA, USA) or SigmaPlot V13 (Systat Software Inc. San Jose, CA, USA). Statistical significance was accepted at the 5% level.