Bd-Infection and Clearance
Each frog was exposed to their respective treatment (see Treatment
Inoculate Preparation ) as a Bd-naïve individual and then again as a
Bd-experienced individual (except for the control treatment groups) to
determine changes in individual behavior. Below we describe the
treatments given to each group and then describe how data were
collected. To create Bd-experienced hosts, individuals were infected
with and cleared of a sublethal dose of live Bd (Fig. 1). Live Bd from
the Bd+ stock was pipetted on the dorsal surface of each amphibian
within their housing chamber (not the experimental chamber) so the Bd
could not be avoided. After exposure, all amphibians (including all
uninfected controls) were then held at 18°C for 11 d, allowing enough
time for growth of the Bd infection but not enough time for Bd to cause
morbidity (McMahon et al. 2014). Amphibians were then cleared of
infections using the heat clearance method described by McMahon,et al. (2014). Briefly, all amphibians were held at 23°C for 3d
(to reduce temperature shock) and were then moved to a 30°C incubator
for 11 d (to clear the infection). Afterward, they were moved to 23°C
for 3 d. before finally returning to 18°C for the remainder of the
experiment (see Fig. 1 for experimental timeline).