Statistical Analysis
Statistics were analyzed with R statistical software (Development Core Team 2013) and significance was attributed at P < 0.05. The greenhouse frogs and pine woods treefrogs were analyzed together because they were conducted simultaneously. Behavioral trials were conducted separately for the Cuban treefrogs and southern toad and thus they were analyzed using independent statistical analyses. For the Cuban treefrog and the southern toad, we conducted general linear mixed effects models (package: nlme, function: lme) with individual amphibian as a random effect (to account for the lack of independence of repeated behavioral observations on an individual), proportion of observations avoiding during a trial as the response variable (arcsine square root-transformed, unfortunately we could not get models with a binomial error distribution to consistently converge), mass as a covariate, and treatment and experience status (naïve versus experienced to Bd, nested within individual as random effects) as crossed independent variables. We followed these analyses with targeted hypothesis testing where we pooled all treatments containing i ) any zoospores, ii ) dead zoospores, and iii ) metabolites in an effort to assess whether any avoidance response was induced by the actual process of infection, physical contact with Bd zoospores, or Bd metabolites, respectively. There were too few greenhouse frogs and pine woods treefrogs to have frogs assigned to treatments with controls solutions assigned to both sides of the container as we did for the Cuban treefrogs and southern toads. Thus, for the greenhouse frogs and pine woods treefrogs, we tested for deviations between the observed and expected null spatial distributions and tested for differences between these two species in their responses because they were tested simultaneously. Probability values were determined using the likelihood ratio tests based on Type II sums of squares (package: car, function: Anova). Amphibians that died during the experiment were excluded from analyses.