Animal Husbandry
Cuban treefrogs (Osteopilus septentrionalis ; adults), southern toads (Bufo terrestris ; metamorphs), greenhouse frogs (Eleutherodactylus planirostris ; adults), and pine woods treefrogs (Hyla femoralis ; metamorphs), were collected within 10 km of the University of Tampa (Tampa, FL). The large arboreal species (Cuban treefrogs) were housed individually in 1-L plastic cups and all other species (pine woods treefrogs, greenhouse frogs and southern toads) were housed in smaller plastic containers (~8 x 11 cm). All animals were maintained with paper towels moistened with artificial spring water (ASW; Cohen et al.1980) and fed gut-loaded crickets dusted with Rep-Cal vitamins ad libitum . Their bedding and containers were changed weekly (new containers were used for each behavioral trial). Animals were maintained at 23°C until the start of the experiment and were then maintained at 18°C unless otherwise noted.