Cuban Treefrog
Cuban treefrogs did not avoid any treatments (Treatment: df = 4, χ2 = 6.66, P = 0.15; Fig. 2A), regardless of whether they were Bd naïve or experienced (experienced: df = 4, χ2 = 0.43, P = 0.51; Treatment*experienced:df = 4, χ2 = 5.25, P = 0.26; Fig. 2B). To increase statistical power, we compared treatments with or without: metabolites (Metabolites: df = 1, χ2 = 0.05,P = 0.82; Metabolites*experience: df =1, χ2 = 4.03, P = 0.05), zoospores (Zoospores:df = 1, χ2 = 1.00, P = 0.32; Zoospores*experience: df = 1, χ2 = 0.02,P = 0.90), and live Bd (Live Bd: χ2 = 0.62,P = 0.43; Live Bd*experience: χ2 = 1.76,P = 0.18). Cuban treefrogs exhibited no evidence of innate or learned avoidance even after pooling these treatments to increase statistical power. Additionally, Cuban treefrogs showed no evidence of avoidance of or attraction to zoospores (dead or alive) or live Bd. Although there was a marginally significant metabolite-by-experience interaction, there was no significant avoidance when naïve or Bd-experienced and experience significantly reduced the strength of the avoidance rather than enhanced it. Thus, this statistical result is not consistent with innate or learned avoidance to Bd.