Cuban Treefrog
Cuban treefrogs did not avoid any treatments (Treatment: df = 4,
χ2 = 6.66, P = 0.15; Fig. 2A), regardless of
whether they were Bd naïve or experienced (experienced: df = 4,
χ2 = 0.43, P = 0.51; Treatment*experienced:df = 4, χ2 = 5.25, P = 0.26; Fig. 2B).
To increase statistical power, we compared treatments with or without:
metabolites (Metabolites: df = 1, χ2 = 0.05,P = 0.82; Metabolites*experience: df =1,
χ2 = 4.03, P = 0.05), zoospores (Zoospores:df = 1, χ2 = 1.00, P = 0.32;
Zoospores*experience: df = 1, χ2 = 0.02,P = 0.90), and live Bd (Live Bd: χ2 = 0.62,P = 0.43; Live Bd*experience: χ2 = 1.76,P = 0.18). Cuban treefrogs exhibited no evidence of innate or
learned avoidance even after pooling these treatments to increase
statistical power. Additionally, Cuban treefrogs showed no evidence of
avoidance of or attraction to zoospores (dead or alive) or live Bd.
Although there was a marginally significant metabolite-by-experience
interaction, there was no significant avoidance when naïve or
Bd-experienced and experience significantly reduced the strength of the
avoidance rather than enhanced it. Thus, this statistical result is not
consistent with innate or learned avoidance to Bd.