Animal Husbandry
Cuban treefrogs (Osteopilus septentrionalis ; adults), southern
toads (Bufo terrestris ; metamorphs), greenhouse frogs
(Eleutherodactylus planirostris ; adults), and pine woods
treefrogs (Hyla femoralis ; metamorphs), were collected
within 10 km of the University of Tampa (Tampa, FL). The large arboreal
species (Cuban treefrogs) were housed individually in 1-L plastic cups
and all other species (pine woods treefrogs, greenhouse frogs and
southern toads) were housed in smaller plastic containers
(~8 x 11 cm). All animals were maintained with paper
towels moistened with artificial spring water (ASW; Cohen et al.1980) and fed gut-loaded crickets dusted with Rep-Cal vitamins ad
libitum . Their bedding and containers were changed weekly (new
containers were used for each behavioral trial). Animals were maintained
at 23°C until the start of the experiment and were then maintained at
18°C unless otherwise noted.