Bd-Infection and Clearance
Each frog was exposed to their respective treatment (see Treatment Inoculate Preparation ) as a Bd-naïve individual and then again as a Bd-experienced individual (except for the control treatment groups) to determine changes in individual behavior. Below we describe the treatments given to each group and then describe how data were collected. To create Bd-experienced hosts, individuals were infected with and cleared of a sublethal dose of live Bd (Fig. 1). Live Bd from the Bd+ stock was pipetted on the dorsal surface of each amphibian within their housing chamber (not the experimental chamber) so the Bd could not be avoided. After exposure, all amphibians (including all uninfected controls) were then held at 18°C for 11 d, allowing enough time for growth of the Bd infection but not enough time for Bd to cause morbidity (McMahon et al. 2014). Amphibians were then cleared of infections using the heat clearance method described by McMahon,et al. (2014). Briefly, all amphibians were held at 23°C for 3d (to reduce temperature shock) and were then moved to a 30°C incubator for 11 d (to clear the infection). Afterward, they were moved to 23°C for 3 d. before finally returning to 18°C for the remainder of the experiment (see Fig. 1 for experimental timeline).