Seasonal Network Reconstruction
Using the PLN models Site name + Water temperature and Site
name , we constructed the species interaction networks for Fall and
Spring, respectively. We tested potential threshold cut-offs for the
networks as depicted in Momal et al. (2020). As there were no
clear thresholds (see Figure S3), we applied a sequence of thresholds
from 0.3–0.7 and generated separate seasonal networks. We limited the
thresholds to 0.3 and above as thresholds below this value created very
dense networks. Specifically, this threshold resulted in species
connecting to, on average, around 40% of their network. As well, we
limited the thresholds to 0.7 and below as thresholds above this value
were too conservative, with many species having no connections. In the
main text, we present seasonal networks constructed with a threshold of
0.5 (Figure 2) as this threshold balanced the trade-offs of over or
under-estimating possible connections (see Supplementary Material for
seasonal networks using the 0.3 and 0.7 thresholds). The subsequent
results for \(\beta\)-diversity and our trait-based analysis were
conducted for the three thresholds (0.3, 0.5, and 0.7) and their results
can be found in the primary text.