Seasonal Network Reconstruction
Using the PLN models Site name + Water temperature and Site name , we constructed the species interaction networks for Fall and Spring, respectively. We tested potential threshold cut-offs for the networks as depicted in Momal et al. (2020). As there were no clear thresholds (see Figure S3), we applied a sequence of thresholds from 0.3–0.7 and generated separate seasonal networks. We limited the thresholds to 0.3 and above as thresholds below this value created very dense networks. Specifically, this threshold resulted in species connecting to, on average, around 40% of their network. As well, we limited the thresholds to 0.7 and below as thresholds above this value were too conservative, with many species having no connections. In the main text, we present seasonal networks constructed with a threshold of 0.5 (Figure 2) as this threshold balanced the trade-offs of over or under-estimating possible connections (see Supplementary Material for seasonal networks using the 0.3 and 0.7 thresholds). The subsequent results for \(\beta\)-diversity and our trait-based analysis were conducted for the three thresholds (0.3, 0.5, and 0.7) and their results can be found in the primary text.