2.2 Cell culture and transfections
Human CC (Cervical Carcinoma) cell lines, HeLa (CCTCC@GDC0009) were obtained from CCTCC (China Center for Type Culture Collection, Wuhan, Hubei, China). HeLa cells were cultured at 37℃ with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), 100 µg/mL streptomycin, and 100 U/mL penicillin. Plasmid transfection of HeLa cells was operated using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. Transfected cells were harvested for RT-qPCR analysis after 48h.