Figure 2. RNA-seq analysis of GNL3-regulated genes in HeLa cells. (A) GNL3 expression quantified by RNA sequencing data. FPKM values were calculated as that has been explained in Materials and Methods. P-value is 0.00441. (B) The result of GNL3 q-RT-PCR analysis. P-value is 4.604E-10. (C) Identification of GNL3 regulated genes. Up-regulated genes are labeled in red, whereas down-regulated are labeled in blue in the heat map. (D) Cumulative distribution of FPKM values of all detected genes in the sample. The horizontal coordinate represents the FPKM value, the vertical coordinate on the left represents the number of genes, and the vertical coordinate on the right represents the proportion of genes. For each point on the curve, its vertical coordinate represents the number and proportion of genes whose FPKM value is less than or equal to the horizontal coordinate of this point. (E) Heat map shows the hierarchically clustered Pearson correlation matrix resulted from comparing the transcript expression values for control and GNL3 knockdown samples.