2.4 Assessment of gene expression
GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was utilized as a control gene for assessing the effects of GNL3 knockdown. cDNA synthesis was done by standard procedures and RT-qPCR was performed on the Bio-Rad S1000 with Bestar SYBR Green RT-PCR Master Mix (DBI Bioscience, Shanghai, China). The information of primers is presented in Table 1. The compression of each transcript was then standardized to GAPDH mRNA level using 2-ΔΔCT method [27]. Comparisons were operated with the paired Student’s t-test by using GraphPad Prism software (San Diego, CA).