a) Total reads: the number of pairs of reads in clean reads, which are used for subsequent analysis.b) Total mapped: the number and proportion of reads that have been mapped to the genome. c) Total mapped reads: the number of reads uniquely located in the reference genome and the proportion of reads in Total mapped reads. d) Total multiple reads: the number of reads with Multiple locations in the reference genome and the proportion of reads in Total mapped reads. Reads with Multiple locations are mainly rRNA and tRNA.