2.4 Assessment of gene expression
GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was utilized as a
control gene for assessing the effects of GNL3 knockdown. cDNA synthesis
was done by standard procedures and RT-qPCR was performed on the Bio-Rad
S1000 with Bestar SYBR Green RT-PCR Master Mix (DBI Bioscience,
Shanghai, China). The information of primers is presented in Table 1.
The compression of each transcript was then standardized to GAPDH mRNA
level using 2-ΔΔCT method [27]. Comparisons were operated with the
paired Student’s t-test by using GraphPad Prism software (San Diego,
CA).