Supplementary Data
Supplementary Table S1 . Primers used for identification of
mutation homozygotes among the progeny of Arabidopsis T-DNA insertionpip mutant lines.
Supplementary Table S2 . Primers used for cloning of potato
aquaporins.
Supplementary Fig. S1 . Identification of mutation homozygotes
among the progeny of Arabidopsis T-DNA insertion pip mutant
lines.
Supplementary Fig. S2 . Effect of nitrogen deficiency on growth
of potato genotypes. Supplementary Fig. S3 . Relative
quantification of Western blot signal for determination of PM
enrichment.
Supplementary Fig S4 . LC-MS based plasma membrane protein
profiling of the two cultivars under N deficiency and control conditions
analyzed by principal component analysis. Supplementary Fig.
S5 . Number of transmembrane helices (TMH) predicted for the identified
proteins.
Supplementary Fig. S6 . Localization of GFP:StPIPs in yeast
cells.
Supplementary Fig. S7 . The ammonium transport ability of PIP1;1
and PIP1;3 studied in yeast by a growth complementation assay (without
ZmPIP2;5 co-expression).
Supplementary Fig. S8 . Correlation between the abundance of PM
ATPase and NRT2.1.