The effect of N deficiency on the growth of potato in vitro
The potato cultivars ‘Lambada’ (sensitive) and ‘Topas’ (tolerant) were
differentiated from a set of potato cultivars according to their
Membership Function Value of stress tolerance calculated from previously
published data (Jozefowicz et al., 2017; Schum et al., 2017). Here, the
root PM proteome was analyzed after seven days of N deficiency. Under
these conditions, the sensitive cultivar displayed a decrease in root
fresh weight, whereas the growth of the tolerant cultivar remained
unaffected. N deficiency at this time point did not lead to differences
in the root N content or in the shoot fresh weights (Supplementary Fig.
S2). This allowed us to analyze mechanisms leading to the early plant
acclimation towards low N, excluding the secondary effects of long-term
N deficiency, such as senescence. However, when the culture duration was
prolonged, the differences between cultivars development and root
architecture were more pronounced (Schum & Jansen, 2012, 2013, 2014).