Supplementary Data
Supplementary Table S1 . Primers used for identification of mutation homozygotes among the progeny of Arabidopsis T-DNA insertionpip mutant lines.
Supplementary Table S2 . Primers used for cloning of potato aquaporins.
Supplementary Fig. S1 . Identification of mutation homozygotes among the progeny of Arabidopsis T-DNA insertion pip mutant lines.
Supplementary Fig. S2 . Effect of nitrogen deficiency on growth of potato genotypes. Supplementary Fig. S3 . Relative quantification of Western blot signal for determination of PM enrichment.
Supplementary Fig S4 . LC-MS based plasma membrane protein profiling of the two cultivars under N deficiency and control conditions analyzed by principal component analysis. Supplementary Fig. S5 . Number of transmembrane helices (TMH) predicted for the identified proteins.
Supplementary Fig. S6 . Localization of GFP:StPIPs in yeast cells.
Supplementary Fig. S7 . The ammonium transport ability of PIP1;1 and PIP1;3 studied in yeast by a growth complementation assay (without ZmPIP2;5 co-expression).
Supplementary Fig. S8 . Correlation between the abundance of PM ATPase and NRT2.1.