Extraction and UPLC-PDA profiling of phenylpropanoids
Phenylpropanoid extraction and detection was performed as described (D.
R. Xu et al., 2019). PDA-detection of phenylpropanoids was performed in
a range between 210 and 800 nm, at a resolution of 1.2 nm and a sampling
rate of 20 points s-1. Spectra for phenylpropanoids
and anthocyanins were recorded at 280 and 520 nm, respectively. Total
phenylpropanoid and anthocyanin contents were quantified as the total
peak area of the compounds detected at 280 and 520 nm, respectively,
eluting between 2 and 7 min. Data analysis was performed using the
software packages Compass Data Analysis V4.4 and QuantAnalysis V4.4
(Bruker Daltonik GmbH, Bremen, Germany). Statistical analysis was
performed using SigmaPlot v13 with a two way ANOVA and Holm-Sidak
post-hoc test.