Microscopy
Yeast cells expressing StPIP1;1 or StPIP1;3 GFP fusion proteins together with either the EV or ZmPIP2;5 were grown in liquid YPG medium. At an optical density at 600 nm of approx. 1, the fluorescence signal was observed. The localization was detected with a Zeiss LSM 780 confocal laser scanning microscope (Carl Zeiss, Jena, Germany) using a 488 nm laser line for excitation. GFP signals were detected at 491-536 nm.