Estimation of fecal calprotectin
Faecal calprotectin was estimated by sandwich ELISA technique using commercially available kits. Fresh stool samples were collected and preserved at -80ÂșC and processed in batches. The degree of colour change was measured through change in optical density. The concentration of faecal calprotectin was estimated using standard plots obtained from standards and controls having known concentration of faecal calprotectin.