Estimation of fecal calprotectin
Faecal calprotectin was estimated by sandwich ELISA technique using
commercially available kits. Fresh stool samples were collected
and preserved at -80ÂșC and processed in batches. The degree of colour
change was measured through change in optical density. The concentration
of faecal calprotectin was estimated using standard plots obtained from
standards and controls having known concentration of faecal
calprotectin.