2.4 Langendorff- perfused isolated hearts
Guinea pigs (male; 300-350 g) were humanely killed by intraperitoneal
injection with pentobarbital sodium (50 mg/kg). Hearts rapidly excised
after thoracotomy were mounted onto a Langendorff perfusion system and
perfused with a modified Tyrode’s solution (119 NaCl, 25
NaHCO3, 4 KCl, 1.2
KH2PO4, 1 MgCl2, 1.8
CaCl2.2H2O, and 10 D-glucose (mM)
equilibrated with 5% CO2 and 95% O2)
with the flow rate of 8 ml/min at 37°C. Hearts were perfused and
monitored for stability for 20 mins before experimental procedures
commenced.