eDNA Metabarcoding
We generated a combined 330,877 sequence reads from 3 eDNA samples, resulting in a total of 2,152 ASVs including singletons; this total dropped to 341 ASVs excluding singletons. Of these 341 ASVs, 211 ASVs were merged paired-reads, 123 ASVs were forward-only reads, and 7 ASVs were reverse only reads. All comparisons below excluded singletons (ASVs that occurred only once across the three samples).