Combined Database vs. Curated Database
The curated California-specific FishCARD database and the combinedCRUX-12S reference database supplemented with the additional California-specific 12S barcodes differed for three taxonomic assignments. First, the curated FishCARD database assigned an ASV to the Black croaker (Cheilotrema saturnum ) that was previously only assigned to the family Sciaenidae by the combined database. Second, the curated FishCARD database identified one ASV as native Bat ray (Myliobatis californica ) whereas the combined database assigned this ASV to the non-native common Eagle ray (Myliobatis aquila ). Third, FishCARD reference database failed to resolve one ASV previously assigned to the family of Delphinidae by the combined database.