Combined Database vs. Curated Database
The curated California-specific FishCARD database and the combinedCRUX-12S reference database supplemented with the additional
California-specific 12S barcodes differed for three taxonomic
assignments. First, the curated FishCARD database assigned an ASV to the
Black croaker (Cheilotrema saturnum ) that was previously only
assigned to the family Sciaenidae by the combined database. Second, the
curated FishCARD database identified one ASV as native Bat ray
(Myliobatis californica ) whereas the combined database assigned
this ASV to the non-native common Eagle ray (Myliobatis aquila ).
Third, FishCARD reference database failed to resolve one ASV previously
assigned to the family of Delphinidae by the combined database.