Reference Barcode Generation
We generated 757 12S MiFish barcodes for 612 California Current
fishes, 557 teleosts, 51 elasmobranchs, and 4 cyclostomatan
(Supplemental Table 2). Of these, 56 barcodes were duplicates of the
same elasmobranch taxa amplified with both the MiFish Elasmobranch and
MiFish Universal 12S primer sets. In total, we generated an
additional 258 novel 12S barcodes for California Current fishes
compared to what was available in GenBank at the time of publication.
Combining these barcodes with existing reference sequences deposited on
GenBank (October 2019) provides reference 12S barcodes for 715
out of 864 (82.8%) California Current fish species.