(3) Perform simulations
In our research, the fu of imipenem was set to 0.8. The formulas were organized in Microsoft Excel software. According to formulas (1) and (6), the imipenem concentration may be assessed after we set thek and V values. The input parameter values included imipenem concentration (at 5 h and 7.5 h), MIC, Tin , τ and dosage. The correlation between Cr and Ca was evaluated (Cr, imipenem concentration detected by UPLC-MS/MS; Ca, imipenem concentration assessed by software). The k and V values may change when the objective function value (OFV) is set to the minimum value. The drug concentration curve was observed, and the result was used when Cr and Ca values coincided. We can obtain f T>MIC from the software.