Expression of target gene has been elevated in microfluidic device
In order to confirm the efficiency of serpentine microfluidic device, comparison of gene expression in two (nucleofection) and three-dimensional system (Microfluidic chip) 24 hours post transfection was performed by Real-Time PCR technique. The mRNA expression of anti-BCMA gene in serpentine device is 33.8 ± 1.7 fold higher than nucleofection (fig.4A). In addition, post hoc analysis of surface protein expression differentiates delivery approaches. Based on results have been showed in fig.4B, our 3D system gene delivery approach was capable of inducing significant expression of anti- BCMA surface protein, three fold, higher than nucleofection method. Taken together, molecular consequences of nucleofection relative to new- generation delivery system highlight the importance of selected delivery strategy for both research and therapeutic applications.