Expression of target gene has been elevated in microfluidic
device
In order to confirm the efficiency of serpentine microfluidic device,
comparison of gene expression in two (nucleofection) and
three-dimensional system (Microfluidic chip) 24 hours post transfection
was performed by Real-Time PCR technique. The mRNA expression of
anti-BCMA gene in serpentine device is 33.8 ± 1.7 fold higher than
nucleofection (fig.4A). In addition, post hoc analysis of surface
protein expression differentiates delivery approaches. Based on results
have been showed in fig.4B, our 3D system gene delivery approach was
capable of inducing significant expression of anti- BCMA surface
protein, three fold, higher than nucleofection method. Taken together,
molecular consequences of nucleofection relative to new- generation
delivery system highlight the importance of selected delivery strategy
for both research and therapeutic applications.