Genetic Analysis
The genomic DNA was extracted by Universal Genomic DNA kit (CWBIO# CWS002). And the exome capture, high throughput sequencing, and standard filtering were performed in the Beijing Novogene Technology Co., Ltd. (Beijing, China, http://www.novogene.com). The genomic DNA samples were randomly fragmented approximately 180 to 280 bp long by Covaris. All exomes were captured by Agilent SureSelect Human All Exon V6 kit and sequenced by Illumina HiSeq Pair End 150 bp platform. The available sequencing data were compared with the reference genome (GRCh37/hg19). The bioinformatics analyses were conducted by Novogene and Mygenostics in Beijing, China. WES indicated gene variants associated with DSD, such as structural variation (SV), single-nucleotide variation (SNV), and insertion and deletions (INDELs).