Generations of knock-down mutants using RNA interfering
technology
To generate RGB1 RNAi transgenic lines, a gene fragment of RGB1 was
amplified from Nipponbare cDNA. A hairpin structure with two inverted
repeat fragments was subsequently constructed and transferred into the
plant binary vector pTCK303, expressed under the control of the maize
ubiquitin promoter (Wang et al., 2004). The construct was transformed
into the Agrobacterium strain EHA105 and used for plant
transformation as described previously (Chen et al. , 2014). The
DNA fragments embracing the targets of the plant transformants were
amplified for sequencing.