Supplemental Figure Legends
Figure S1. Culture of S. aureus in chambers with different volumes in the presence and absence of A. graevenitzii. A-D) Chamber designs with a 200 µm outer chamber and inner chambers of 10 µm (A), 30 µm (B), 50 µm (C), and 100 µm (D). E) Growth of S. aureus in chambers of different height, in the presence (upper panels) and absence (lower panels) of A. graevenitzii FO582 compared to growth observed in the 50 µm design (Fig. 1). Growth of S. aureus does not cover the entire area in chambers with heights lower than 50 µm. F) Graph shows measurement of S. aureus coverage in the presence and absence of A. graevenitzii in different height chambers. No difference is observed in shallow chambers where S. aureus growth is already restricted by the small volume. Comparison of 50 and 100 µm high chambers demonstrated that smaller exclusion zones are generated in larger volume co-cultures. Error bars: mean ± SD, n ≥ 5 chambers scored per condition.
Figure S2. Co-culture of actinomyces and schaalia with streptococcal species in microchambers. Representative images from microfluidic co-culture experiments, supplemental to Figure 2A. Scale bar: 50 µm.
Figure S3. Macroscopic co-culture of actinomyces and schaalia species with S. aureus. A) Spot monocultures of actinomyces and schaalia species on BHI agar plates. 2/18 strains failed to grow. B) Co-culture of actinomyces or schaalia species with Staphylococcus aureus (Strain SH1000-GFP) did not show macroscopic cross-species inhibition.