Expressions of AtMTM1, AtMTM2, and AtMSD1under metal stresses
To elucidate the effect of metal stress on AtMTM1 , AtMTM2 ,
and AtMSD1 gene regulations, we transferred 2-week-old seedlings
to buffers containing 100 μM of Mn (MnCl2), Fe (Fe
citrate), Cu (CuSO4), Zn (ZnSO4), Mg
(MgSO4), and Ca (CaCl2) for 24 h with
gentle shaking (Figure 6) .
Compared to the unstressed condition, AtMTM1 , AtMTM2 , andAtMSD1 expressions responded positively to most metal stresses by
qPCR. The 100 μM Mn treatment caused no significant effect onAtMTM1 expression. AtMTM2 was markedly responsive to Cu
stress compared with AtMTM1 and AtMSD1 . Generally, all
three genes were up-regulated synchronically under metal stresses.