Rescue of mtm1-i, mtm2, and mtm1-i mtm2root phenotype under Mn supplemental condition
To clarify the defective metal regulation in transporter mutants,
researchers usually applied metal supplementation and observed
phenotypic complementation (Eisenhuta et al., 2018; Schneider et al.,
2016). The 1/2 MS basal salt mixture contained 50 μM Mn and other
required nutrients. The root lengths of mtm1-i , mtm2 , andmtm1-i mtm2-#1 (referred as mtm1-i mtm2 ) were monitored
on 1/2 MS plate without or with additional 10 to 500 μM
MnCl2 for 6 d (Figure 10) .
Without additional Mn treatment, we observed the root length of 6-d-oldmtm1-i seedlings was shorter; the mtm2 and mtm1-i
mtm2 were longer than the WT. The root length of mtm1-i was
recovered on additional 10 and 50 μM Mn-containing plates compared with
the WT. The extra 500 μM Mn supplement rescued the root length to the WT
in all single and double mutants. Overall, the additional Mn supplement
revealed the complementary effect of the root length in single and
double mutants.