Yeast strains and growth condition
Yeast (Saccharomyces cerevisiae ) used in this study contained the WT BY4741 (MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 ), ysod2Δ(sod2::kanMX4 ), and ymtm1Δ (mtm1::LEU2 MC101) mutants. Yeasts were incubated at 30°C under aerobic condition (Brachmann et al., 1998). Enriched yeast extract-peptone-dextrose (YPD) media was supplemented with 2% Glc, and synthetic dextrose (SD) medium was used to culture yeast strains. For yeast expression, all cDNAs were cloned into the yeast expression vector pADNS. Yeast transformation involved the lithium acetate procedure (Gietz & Schiestl, 1991), and transformants were selected on minimal SD media. Yeast was incubated at 30°C under the aerobic condition without shaking, and G418 was used at 200 μg mL-1 to maintain ySOD2 and yMTM1deletion mutants of ysod2Δ and ymtm1Δ . For plate assay, yeast cultures were diluted serially from A 600 = 1 to 10-4. Yeast transformants were plated without or with MV, and incubated at 30°C under the aerobic condition for 10 d.