Constructs of organelle-destined AtMSD1
The deletion of the mitochondrial transit peptide of AtMSD1 resulted in cytosol-localized AtMSD1. The mitochondrial transit peptide of AtMSD1 that was replaced by the chloroplast transit peptide of CSD2 (Huang et al., 2012b) became chloroplast-destined AtMSD1. Besides, a tag of 15-amino acids or 3xFLAG was fused to the C-terminus of AtMSD1 to distinguish the exogenous-expressed AtMSD1 from endogenous AtMSD1.