Introduction.
IS1 sequence is found 8 copies in Escherichia coli k-12. IS1…..IS10 are mainly existing as flanking gene in repeats and inverted repeats on both sides of transposable elements, characterised by Tn3 as Ampicillin, Tn5 as Chloramphenicol, and Tn10 as Tetracycline.[1-4,5-7] They are responsible for causing mutation, antibiotic resistance and pathogenesis. Radioisotope labelled IS1, IS2 and IS3 DNA sequence probes with 0.8kb to 1.2 kb, DNA sequence could support the process quickly and accurately. Bacteria, Fungi, Mould and Algae are belonging to organised (eukaryotic) nucleic acid cells. Our body cell system belongs to eukaryotic cells and tissues. Viral cells are different. Viral cells as prokaryon, unorganized (prokaryotic), carry RNA and DNA ss, (single stranded), or ds (double stranded) coated by protein. They are nano and micron level big and are air-, dust-, insect-vector-and water- born. Their functionality will depend on host, their survivals depend on host. Based on host specificity, they can be living and pathogen; otherwise they get abolished in the long run. For survivals virus change their functions by mutations to sustain in evolution. All prokaryotic and virus have partial and full parasitic nature. It proves that DNA could have enormous power to adapt the environment. In support of bacteriophages (like Lambda, Psi-ex, T4 viruses) specific to bacteria, they maintain their existence in different animal viruses. Like. Adenoid-, retro- and rhinoviruses, specific to human, animal, birds and insect for host specific growth and transmission, [5,6]. Virus cannot survive long, in scientific point of view, since they have no cell wall, only by protein and lipid coat, they remain dormant inactive. By epigenetic manipulation viruses manipulate single (ss) and double stranded (ds) DNARNAProtein propagations in host specific animal cells. As per the laws of Darwin and in the thrust of survivals, they aggressively search host, as non-infective, long and short term infections,[7-10]. Spanish flue, Influenza, MARS, SARS,HIV are those examples.IS1, IS2 and IS3, or other Tn Sequence, if found, could be used as DNA short sequence probe and could be used to develop antibodies, specific to corona MERS, SARS, HIV viruses. Methods of probe-DNA isolation have been discussed, [1-5, 7-10].Although the mode of infection and transmission of these viruses are different, namely, air, dust,-water, human and animal born, mutations have accomplished them to adapt and to search matrix/ vector as carrier (i.e. mosquito born Dengue) in Asia/India migrated from USA, Latino America and South East Asia, different than corona. . The most important part of these viruses is the coat (protein or lipid) and their epigenetic function of RNA and DNA, supported by ss, ds, replication, transcription and translations. The mode of Infectivity, rapidity, and sustainability of these viruses are basically based on such coats and by their adaptive mutations of the host cells, their propagations, long and short distance. Viruses are cleverer, compared to bacteria. For designing vaccine against such infective agents, IS mediated hybridization could be essential to classify them in adeno (DNA) and retro (RNA) viruses, including corona. By cloning HA gene of corona intoE. coli , the surface antigen of hybrid E.colifimbriae/pili could be used for humoral memory based response of T- and B-cells and as cytotoxic Tc and Tk cells. Since IS could be available both in mutations and in wild types, [1-7], evolution of virus is supposed to be caused by the fragments of nucleic acids of the host cells, Fig.1-5].