Fig.7 Fig.8 Fig.9.
IS mediated viral spike gene cloning has been proposed and reproduced in following manner by the author. IS probe was isolated purified from the known source of E.coli k-12 .The same probe could be used to search the presence of IS in corona, SARS, MERS, HIV, Influenza viruses to short them in groups. IS(Insertion Sequence) DNA probes were p32 alfa d ATP labelled as ss stranded DNA, and were hybridized against ss DNA, and ss RNA of viruses. Southern and Northern blotting both could be used as per the need, Fig.9. Prof. Barbara Mc Clintok of USA, discovered Tn in Maize seeds by their segregated color changes and named Tn ( Transposable element) Later Prof. P.Starlinger studied the same in Bacteria E.coli , Prof. H. Saedler as his follower continued the same. The integration and multiplications/ jumping from one place to other, from one gene/ plasmid to other could be initiated by IS elements and their repeated and inverted repeat sequences. The searching of IS element in corona could similarly be made as proposed. DNA of hybrid strains would be studied separately. By isolating their chromosome DNA and Plasmids, the presence of copies of IS element was studied. 8 copies of IS1 and number of copies of other IS elements were varried including the sequence lengths. The changing the number of IS copies in hybrid chromosome and plasmid will confirm, the cloning of viral spike gene by copies of IS elemens, if compared with IS copies of the original recipient E.coli k-12 C600 Yale strain, already existing with 8 copies of IS1 in E.coli chromosome.. In case of hybrid these copies will remain same or will change, Fig.10.