Introduction.
IS1 sequence is found 8 copies in Escherichia coli k-12.
IS1…..IS10 are mainly existing as flanking gene in repeats and
inverted repeats on both sides of transposable elements, characterised
by Tn3 as Ampicillin, Tn5 as Chloramphenicol, and Tn10 as
Tetracycline.[1-4,5-7] They are responsible for causing
mutation, antibiotic resistance and pathogenesis. Radioisotope labelled
IS1, IS2 and IS3 DNA sequence probes with 0.8kb to 1.2 kb, DNA sequence
could support the process quickly and accurately. Bacteria, Fungi, Mould
and Algae are belonging to organised (eukaryotic) nucleic acid cells.
Our body cell system belongs to eukaryotic cells and tissues. Viral
cells are different. Viral cells as prokaryon, unorganized
(prokaryotic), carry RNA and DNA ss, (single stranded), or ds (double
stranded) coated by protein. They are nano and micron level big and are
air-, dust-, insect-vector-and water- born. Their functionality will
depend on host, their survivals depend on host. Based on host
specificity, they can be living and pathogen; otherwise they get
abolished in the long run. For survivals virus change their functions by
mutations to sustain in evolution. All prokaryotic and virus have
partial and full parasitic nature. It proves that DNA could have
enormous power to adapt the environment. In support of bacteriophages
(like Lambda, Psi-ex, T4 viruses) specific to bacteria, they maintain
their existence in different animal viruses. Like. Adenoid-, retro- and
rhinoviruses, specific to human, animal, birds and insect for host
specific growth and transmission, [5,6]. Virus cannot
survive long, in scientific point of view, since they have no cell wall,
only by protein and lipid coat, they remain dormant inactive. By
epigenetic manipulation viruses manipulate single (ss) and double
stranded (ds) DNARNAProtein propagations in host specific animal cells.
As per the laws of Darwin and in the thrust of survivals, they
aggressively search host, as non-infective, long and short term
infections,[7-10]. Spanish flue, Influenza, MARS, SARS,HIV
are those examples.IS1, IS2 and IS3, or other Tn Sequence, if found,
could be used as DNA short sequence probe and could be used to develop
antibodies, specific to corona MERS, SARS, HIV viruses. Methods of
probe-DNA isolation have been discussed, [1-5, 7-10].Although the mode of infection and transmission of these viruses are
different, namely, air, dust,-water, human and animal born, mutations
have accomplished them to adapt and to search matrix/ vector as carrier
(i.e. mosquito born Dengue) in Asia/India migrated from USA, Latino
America and South East Asia, different than corona. . The most important
part of these viruses is the coat (protein or lipid) and their
epigenetic function of RNA and DNA, supported by ss, ds, replication,
transcription and translations. The mode of Infectivity, rapidity, and
sustainability of these viruses are basically based on such coats and by
their adaptive mutations of the host cells, their propagations, long and
short distance. Viruses are cleverer, compared to bacteria. For
designing vaccine against such infective agents, IS mediated
hybridization could be essential to classify them in adeno (DNA) and
retro (RNA) viruses, including corona. By cloning HA gene of corona intoE. coli , the surface antigen of hybrid E.colifimbriae/pili could be used for humoral memory based response of T- and
B-cells and as cytotoxic Tc and Tk cells. Since IS could be available
both in mutations and in wild types, [1-7], evolution of
virus is supposed to be caused by the fragments of nucleic acids of the
host cells, Fig.1-5].