NG2-glia morphological alteration in L-DOPA-induced
dyskinesia.
The number of branches, their length (µm), the number of junctions and
the soma area (µm2) of NG2-glia were quantified in
each of the four subdivisions of striatum (Fig. 3).
Firstly, the ramification index of the NG2-glia after L-DOPA chronic
treatment of parkinsonian rats and doxycycline administrationincreased in the dorsolateral striatum, decreased in the
dorsomedial and ventromedial striata, and presented no changes in the
ventrolateral division (Figs A, A”, B-E) when compared to rats that
received only L-DOPA treatment.
Animals receiving L-DOPA treatment and exhibiting LID presenteda decrease in the number of branches per cell and the average
of the branch length in the dorsolateral striatum compared to the 6OHDA
lesioned animals. In the dorsomedial and ventromedia l striatum
L-DOPA treatment-induced, per cell, an increase in the number of
branches, the average branches length, and the number of junctions
compared to 6OHDA lesioned animals. The dorsomedial striatumalso showed an increase in the average of the soma area. There was a
decrease in NG2-glia cells index activation in the dorsolateral striatum
of rats presenting LID (Fig 3).
In the dorsolateral striatum of the animals presenting LID and
treated with doxycycline was revealed an increased NG2-glia pattern of
activation as evaluated by the number and the average branches length
per cell, the number of junctions per cell (entangled with each other)
and the average soma area when compared to 6OHDA lesion and6OHDA+Vehicle+L-DOPA groups. There was a decrease in the NG2-glia
indicators of activation in the dorsomedial and ventromedial
striatum.
Doxycycline treatment decreased dyskinesia and the signals of the
NG2-glia activation in the dorsomedial and ventromedial
striatum (the number of branches per cell and the average branch
length). In the dorsomedial striatum it was also observed a decrease in
the NG2-glia average of the soma area compared to 6OHDA+L-DOPAgroup.
There were no changes in the NG2-glia cells measured parameters in the
ventrolateral striatum in all experimental groups (Nolesion (control), 6OHDA lesion, 6OHDA+DOXY, 6OHDA+Vehicle+ L-DOPA
and 6OHDA+L-DOPA+DOXY ).